A new computational method for detection of chimeric 16S rRNA artifacts generated by PCR amplification from mixed bacterial populations
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Summary
This study suggests that k-tuple-based matching methods are more sensitive than alignment-based methods when there is significant parental sequence similarity, while the opposite becomes true as the sequences become more distantly related.
- Type
- article
- Published
- 1997-06-01
- Cited by
- 57
- References
- 24
- Access
- Open access
- OpenAlex
- https://openalex.org/W1791996297
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:13379616
Keywords
Chimera (genetics), Computational biology, Bacterial artificial chromosome, Sequence alignment, Biology
References
- Sequence alignment and penalty choice. Review of concepts, case studies and implications.
- Evaluation of nearest-neighbor methods for detection of chimeric small-subunit rRNA sequences
- Recognition of chimeric small-subunit ribosomal DNAs composed of genes from uncultivated microorganisms
- Selective Recovery of 16S rRNA Sequences from Natural Microbial Communities in the Form of cDNA
- The phylogeny of prokaryotes.
- Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment.
- Parametric sequence comparisons.
- Improved tools for biological sequence comparison.
- Identification in situ and phylogeny of uncultured bacterial endosymbionts
- 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community
- Basic local alignment search tool.
- Genetic diversity in Sargasso Sea bacterioplankton
- Chimeric alignment by dynamic programming: algorithm and biological uses
- A general method applicable to the search for similarities in the amino acid sequence of two proteins.
- Identification of common molecular subsequences.
- Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis
- Phylogenetic diversity of subsurface marine microbial communities from the Atlantic and Pacific Oceans
- Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya.
- The Ribosomal Database Project.
- A Fast Filtration Algorithm for the Substring Matching Problem
Cited by
- Microbial biodiversity in Tasmanian caves
- Elucidation of the Microbial Consortium of the Ubiquitous Shore Sponge, Tedania ignis
- Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
- A jumping profile Hidden Markov Model and applications to recombination sites in HIV and HCV genomes
- Sequences, the environment and fungi
- Phylogenetic Analyses of Archaeal Ribosomal DNA Sequences from Salt Pan Sediment of Mumbai, India
- Proposals for classification methods dedicated to biological data
- Automatic identification of large collections of protein-coding or rRNA sequences.
- Molecular characterization of bacterial population in the forest soil of Kashmir, India
- A molecular microbial ecology approach to studying hemodialysis water and fluid.
- Resequencing a Set of Strings Based on a Target String
- Phylogenetic Characterization of Archaea in Saltpan Sediments
- Phylogenetic analysis of bacterial communities associated with ectoparasitic chewing lice of pocket gophers: A culture-independent approach
- Diversity In Symbiotic Dinoflagellates (Pyrrhophyta) from Seven Scleractinian Coral Species: Restriction Enzyme Analysis of Small Subunit Ribosomal RNA Genes
- PCR-Induced Sequence Artifacts and Bias: Insights from Comparison of Two 16S rRNA Clone Libraries Constructed from the Same Sample
- Microvariation Artifacts Introduced by PCR and Cloning of Closely Related 16S rRNA Gene Sequences
- Molecular Evidence for Association of Chlamydiales Bacteria with Epitheliocystis in Leafy Seadragon (Phycodurus eques), Silver Perch (Bidyanus bidyanus), and Barramundi (Lates calcarifer)
- Sequence Analysis of Marine Virus Communities Reveals that Groups of Related Algal Viruses Are Widely Distributed in Nature
- Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons.
- The double helix meets the crystal lattice: The power and pitfalls of nucleic acid approaches for biomineralogical investigations
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