Subtle gene modification in mouse ES cells: evidence for incorporation of unmodified oligonucleotides without induction of DNA damage
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Summary
The results demonstrate that the use of unmodified rather than PTO end-protected ssODNs allows stable gene modification without compromising the genomic integrity of the cell, which is crucial for application of ssODN-mediated gene targeting in (embryonic) stem cells.
- Type
- article
- Published
- 2010-07-02
- Cited by
- 33
- References
- 29
- Access
- Open access
- OpenAlex
- https://openalex.org/W2077057652
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:8813168
Keywords
Biology, Sense (electronics), Oligonucleotide, Gene, Embryonic stem cell
References
- Cellular responses to targeted genomic sequence modification using single-stranded oligonucleotides and zinc-finger nucleases.
- The comet assay: a method to measure DNA damage in individual cells
- Stable transmission of targeted gene modification using single-stranded oligonucleotides with flanking LNAs
- Targeted gene repair activates Chk1 and Chk2 and stalls replication in corrected cells.
- DNA Double-stranded Breaks Induce Histone H2AX Phosphorylation on Serine 139*
- DNA breakage associated with targeted gene alteration directed by DNA oligonucleotides
- HYDROXYUREA: A SPECIFIC INHIBITOR OF DEOXYRIBONUCLEIC ACID SYNTHESIS.
- A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage.
- Use of internally nuclease‐protected single‐strand DNA oligonucleotides and silencing of the mismatch repair protein, MSH2, enhances the replication of corrected cells following gene editing
- DNA Replication, cell cycle progression and the targeted gene repair reaction
- Implications of cell cycle progression on functional sequence correction by short single-stranded DNA oligonucleotides
- Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression.
- Gene repair in mammalian cells is stimulated by the elongation of S phase and transient stalling of replication forks.
- Effective oligonucleotide-mediated gene disruption in ES cells lacking the mismatch repair protein MSH3
- Oligonucleotide-Mediated Gene Targeting in Human Hepatocytes: Implications of Mismatch Repair
- Generation of Destabilized Green Fluorescent Protein as a Transcription Reporter*
- Regulation of targeted gene repair by intrinsic cellular processes
- Genomic sequence correction by single‐stranded DNA oligonucleotides: role of DNA synthesis and chemical modifications of the oligonucleotide ends
- Parameters of oligonucleotide-mediated gene modification in mouse ES cells
- Targeted gene modification in mismatch-repair-deficient embryonic stem cells by single-stranded DNA oligonucleotides.
Cited by
- GUIDE-Seq to Detect Genome-wide Double-Stranded Breaks in Plants.
- Oligonucleotide Delivery by Nucleofection Does Not Rescue the Reduced Proliferation Phenotype of Gene-Edited Cells
- Oligo/Polynucleotide-Based Gene Modification: Strategies and Therapeutic Potential
- Progress and prospects: oligonucleotide-directed gene modification in mouse embryonic stem cells: a route to therapeutic application
- Precise Gene Modification Mediated by TALEN and Single-Stranded Oligodeoxynucleotides in Human Cells
- Stable Gene Targeting in Human Cells Using Single-Strand Oligonucleotides with Modified Bases
- High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases
- Transient suppression of MLH1 allows effective single-nucleotide substitution by single-stranded DNA oligonucleotides.
- Replicative DNA Polymerase δ but Not ε Proofreads Errors in Cis and in Trans
- Transformation with Oligonucleotides Creating Clustered Changes in the Yeast Genome
- Emerging gene editing strategies for Duchenne muscular dystrophy targeting stem cells
- Toward Multiplex Genome Engineering in Mammalian Cells
- DNA Damage Response Pathway and Replication Fork Stress During Oligonucleotide Directed Gene Editing
- Oligonucleotide-directed gene-editing technology: mechanisms and future prospects
- Use of the HPRT gene to study nuclease-induced DNA double-strand break repair
- LNA modification of single-stranded DNA oligonucleotides allows subtle gene modification in mismatch-repair-proficient cells
- Enhanced gene targeting to evaluate Lynch syndrome alterations
- Frontiers in Skeletal Muscle Wasting, Regeneration and Stem Cells
- Comprehensive protocols for CRISPR/Cas9-based gene editing in human pluripotent stem cells
- A ‘Semi-Protected Oligonucleotide Recombination’ Assay for DNA Mismatch Repair in vivo Suggests Different Modes of Repair for Lagging Strand Mismatches
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