A test-bed for optimizing high-resolution single particle reconstructions.
Explore this paper's citation graph
Summary
Using GroEL as a test-bed, a series of 3D reconstructions where the number of particles used in computing the map, the accelerating voltage of the microscope, and the electron dose used to acquire the images resulted in a map with a nominal resolution of 5.4A.
- Type
- article
- Published
- 2008-07-01
- Cited by
- 35
- References
- 33
- Access
- Open access
- OpenAlex
- https://openalex.org/W2055515293
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:35044489
Keywords
Particle (ecology), Test (biology), Resolution (logic), Computer science, Geology
References
- Lipid–protein interactions in double-layered two-dimensional AQP0 crystals
- Ab initio resolution measurement for single particle structures.
- Exact filters for general geometry three dimensional reconstruction
- Optimization of image collection for cellular electron microscopy.
- Particle finding in electron micrographs using a fast local correlation algorithm.
- Noise bias in the refinement of structures derived from single particles.
- Automated cryoEM data acquisition and analysis of 284742 particles of GroEL.
- EMAN: semiautomated software for high-resolution single-particle reconstructions.
- Conformational variability in the refined structure of the chaperonin GroEL at 2.8 Å resolution
- Structure of keyhole limpet hemocyanin type 1 (KLH1) at 15 A resolution by electron cryomicroscopy and angular reconstitution.
- Near-atomic resolution using electron cryomicroscopy and single-particle reconstruction
- ATP-bound states of GroEL captured by cryo-electron microscopy.
- Visualization of a 4-helix bundle in the hepatitis B virus capsid by cryo-electron microscopy
- Fourier shell correlation threshold criteria.
- A relational database for cryoEM: experience at one year and 50 000 images.
- Advantages of CCD detectors for de novo three-dimensional structure determination in single-particle electron microscopy.
- Automated molecular microscopy: the new Leginon system.
- Seeing GroEL at 6 A resolution by single particle electron cryomicroscopy.
- Allosteric signalling of ATP hydrolysis in GroEL-GroES complexes
- Determination of the fold of the core protein of hepatitis B virus by electron cryomicroscopy
Cited by
- Cryo-Electron Microscopy - Estimating Conformational Variances by Principal Motion Analysis
- 4.6 Å Cryo-EM reconstruction of tobacco mosaic virus from images recorded at 300 keV on a 4k × 4k CCD camera
- Strategies to Resolve the Three-Dimensional Structure of the Genome of Small Single-Stranded Icosahedral Viruses
- Single-particle cryoEM analysis at near-atomic resolution from several thousand asymmetric subunits.
- Adeno-Associated Virus-2 and its Primary Cellular Receptor – Cryo-EM Structure of a Heparin Complex
- Initial evaluation of a Direct Detection Device detector for single particle cryo-electron microscopy
- Maximum likelihood refinement of electron microscopy data with normalization errors
- Validation of 3D EM Reconstructions: The Phantom in the Noise
- A helical processing pipeline for EM structure determination of membrane proteins
- Creating an Infrastructure for High-Throughput High-Resolution Cryogenic Electron Microscopy
- A novel approximation method of CTF amplitude correction for 3D single particle reconstruction.
- Specific arrangement of alpha-helical coiled coils in the core domain of the bacterial flagellar hook for the universal joint function.
- DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy
- Hydration-layer models for cryo-EM image simulation
- High-resolution single-particle orientation refinement based on spectrally self-adapting common lines.
- ResLog Plots as an Empirical Metric of the Quality of Cryo-EM Reconstructions
- Cryomesh™: A new substrate for cryo-electron microscopy
- PRIME: probabilistic initial 3D model generation for single-particle cryo-electron microscopy.
- A Bayesian method for 3D macromolecular structure inference using class average images from single particle electron microscopy
- Image processing and computing in structural biology
Related papers
- Effects of SiC contents on the microstructure and mechanical properties of Al4SiC4–SiC ceramic composites
- Porous SiC Ceramics with Multiple Pore Structure Fabricated via Gelcasting and Solid State Sintering
- Effects of Graphene Transfer and Thermal Annealing on Anticorrosive Properties of Stainless Steel
- Effect of SiC on Mechanical and Microstructural Characteristics of Al Based Functionally Graded Material
- Fabrication of (TiB/Ti)-TiAl composites with a controlled laminated architecture and enhanced mechanical properties
- Pressure sintering of W-15wt.%Cu alloys prepared by mechanical alloying as a subsequent densification treatment method
- Additively manufactured SiC-reinforced stainless steel with excellent strength and wear resistance