Current two‐dimensional electrophoresis technology for proteomics
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Summary
The current 2‐DE/MS workflow is described including the following topics: sample preparation, protein solubilization, and prefractionation; protein separation by 1‐DE with IPGs; protein detection and quantitation; computer assisted analysis of 2-DE patterns; protein identification and characterization by MS; two‐dimensional protein databases.
- Type
- review
- Published
- 2004-12-01
- Cited by
- 1,845
- References
- 164
- OpenAlex
- https://openalex.org/W2048333869
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:28594824
Keywords
Proteomics, Chemistry, Chromatography, Mass spectrometry, Isoelectric point
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- Capillary electrophoresis-mass spectrometry for Peptide analysis: target-based approaches and proteomics/peptidomics strategies.
- Proteomic expression profiling of breast cancer.
- Multidimensional protein identification technology (MudPIT): Technical overview of a profiling method optimized for the comprehensive proteomic investigation of normal and diseased heart tissue
- Marked defects in the expression and glycosylation of alpha2-HS glycoprotein/fetuin-A in plasma from neonates with intrauterine growth restriction: proteomics screening and potential clinical implications.
- Plant protein isolation and stabilization for enhanced resolution of two-dimensional polyacrylamide gel electrophoresis.
- Fasting status as a consideration for human serum collection and preparation prior to depletion and analysis
- Two-dimensional gel electrophoresis of wool intermediate filament proteins.
- Analysis of high molecular mass proteins larger than 150 kDa using cyanogen bromide cleavage and conventional 2‐DE
- Using cell engineering and omic tools for the improvement of cell culture processes
- IgG glycosylation analysis
- Proteomic strategies and challenges in tumor metastasis research
- Linking protein oxidation to environmental pollutants: redox proteomic approaches.
- Cardiovascular proteomics: tools to develop novel biomarkers and potential applications.
- A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots
- 2‐D Gel‐Based Proteomic Approaches to Antibiotic Drug Discovery
- Immunoproteomics of Brucella abortus RB51 as candidate antigens in serological diagnosis of brucellosis.
- Proteomic Challenges: Sample Preparation Techniques for Microgram-Quantity Protein Analysis from Biological Samples
- Cloning, expression, and antigenic characterization of recombinant protein of Mycoplasma gallisepticum expressed in Escherichia coli.
- Proteomics in obesity research
- Transformation of plum plants with a cytosolic ascorbate peroxidase transgene leads to enhanced water stress tolerance.
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