Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250
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Summary
An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G‐250 and R‐250 to combine the advantage of much shorter staining time with high sensitivity, a clear background not requiring destaining, stepwise staining, and stable fixation after staining.
- Type
- article
- Published
- 1988-01-01
- Cited by
- 2,662
- References
- 6
- OpenAlex
- https://openalex.org/W1964313477
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:27062618
Keywords
Staining, Isoelectric focusing, Coomassie Brilliant Blue, Polyacrylamide, Chromatography
References
- A "high-performance" 2D gel scanner.
- Isoelectric focusing in gels.
- Visualization of proteins with a silver “stain”: A critical analysis
- Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis
- One‐ and two‐dimensional electrophoresis in micro‐slab gels
- Improved design of one‐dimensional vertical polyacrylamide slab gel electrophoresis avoids lateral streaking
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