Mitochondria‐targeted green fluorescent proteins: convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae
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Summary
A novel set of plasmids for expression of mitochondria‐targeted green fluorescent protein (GFP) in Saccharomyces cerevisiae facilitate convenient analysis of mutants defective in mitochondrial morphology or inheritance and are suitable vectors for easy construction of different kinds of GFP fusion proteins to study various aspects of organelle biogenesis in living yeast cells.
- Type
- article
- Published
- 2000-11-01
- Cited by
- 422
- References
- 35
- OpenAlex
- https://openalex.org/W2033062319
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:3228544
Keywords
Green fluorescent protein, Biology, Mitochondrion, Organelle, Yeast
References
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- Improved method for high efficiency transformation of intact yeast cells.
- The Dynamin-related GTPase, Dnm1p, Controls Mitochondrial Morphology in Yeast
Cited by
- Mdm30 is an F-box protein required for maintenance of fusion-competent mitochondria in yeast.
- Importance of mitochondrial dynamics during meiosis and sporulation.
- Short tetracysteine tags to beta-tubulin demonstrate the significance of small labels for live cell imaging.
- Mdv1 Interacts with Assembled Dnm1 to Promote Mitochondrial Division*
- Nucleoid localization of Hsp40 Mdj1 is important for its function in maintenance of mitochondrial DNA
- The deafness gene DFNA5 induces programmed cell death through mitochondria and MAPK-related pathways
- A role for Saccharomyces cerevisiae Centrin (Cdc31) in mitochondrial function and biogenesis
- INVESTIGATING THE ROLES OF REACTIVE OXYGEN AND NITROGEN SPECIES IN PLANT PROGRAMMED CELL DEATH, CYTOSKELETAL AND MITOCHONDRIAL DYNAMICS
- Fusion PCR via novel overlap sequences.
- Live-cell imaging of the cytoskeleton and mitochondrial-cytoskeletal interactions in budding yeast.
- Determination of yeast mitochondrial KHE activity, osmotic swelling and mitophagy.
- Involvement of mitochondrial proteins in yeast apoptosis
- Minimum GC-rich sequences for overlap extension PCR and primer annealing.
- Identification of cellular functions of cardiolipin as physiological modifiers of barth syndrome
- Saccharomyces cerevisiae as a model organism to study mitochondrial biology: general considerations and basic procedures.
- Evidence that the antiproliferative effects of auranofin in Saccharomyces cerevisiae arise from inhibition of mitochondrial respiration.
- Etude de deux gènes impliqués dans la biosynthèse du parfum chez le genre Rosa L. (Rosaceae)
- Fluorescence nanoscopy in three dimensions
- The dynamic status of actin in the regulation of environmental sensing and homeostatic control in Saccharomyces cerevisiae
- Proteome and metabolome profiling of wild-type and YCA1-knock-out yeast cells during acetic acid-induced programmed cell death.
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