Thermosensitivity of green fluorescent protein fluorescence utilized to reveal novel nuclear-like compartments in a mutant nucleoporin NSP1.
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Summary
Proteins synthesized at a low temperature retained their fluorescence despite a shift to a higher temperature, and when a temperature-sensitive nsp1 mutant expressing GFP-nucleoplasmin was cultured at 23 degrees C and then shifted to 35 degrees C, this protein accumulated in novel nuclear-like compartments devoid of DNA.
- Type
- article
- Published
- 1995-07-01
- Cited by
- 93
- References
- 0
- OpenAlex
- https://openalex.org/W1789456031
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:46211095
Keywords
Nucleoporin, Fluorescence, Green fluorescent protein, Mutant, Cell biology
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