Highly efficient selection of phage antibodies mediated by display of antigen as Lpp-OmpA' fusions on live bacteria.
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Summary
Evaluation of those phage that display a specific antibody over phages that display an irrelevant antibody of over 1,000,000 in a single DIP cycle is demonstrated.
- Type
- article
- Published
- 2000-08-25
- Cited by
- 39
- References
- 44
- OpenAlex
- https://openalex.org/W2026057748
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:24425343
Keywords
Phage display, Phagemid, Panning (audio), Antibody, Peptide library
References
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- Selectively infective phage (SIP) technology: scope and limitations.
- Organ targeting In vivo using phage display peptide libraries
- Energy‐linked oxidation of glutaraldehyde by rat liver mitochondria
- MUC1 isoform specific monoclonal antibody 6E6/2 detects preferential expression of the novel MUC1/Y protein in breast and ovarian cancer
- Selective phage infection mediated by epitope expression on F pilus.
- Biotechnological applications of phage and cell display.
Cited by
- Recombinant single chain antibodies in bioelectrochemical sensors.
- Single chain antibodies that recognize the N-glycosylation site.
- Genetically Engineered Microbial Fuel Cells
- Humanization and epitope mapping of the H23 anti-MUC1 monoclonal antibody reveals a dual epitope specificity.
- Inhibition of hepatitis C virus NS3-mediated cell transformation by recombinant intracellular antibodies.
- Design of synthetic antibody libraries
- Synthetic antibodies: concepts, potential and practical considerations.
- HCV NS3 serine protease-neutralizing single-chain antibodies isolated by a novel genetic screen.
- Peptide mimotopes recognized by antibodies cetuximab and matuzumab induce a functionally equivalent anti-EGFR immune response
- Antibody isolation from immunized animals: comparison of phage display and antibody discovery via V gene repertoire mining.
- Biotechnological applications of phage and cell display.
- Recombinant immunotoxins and retargeted killer cells: employing engineered antibody fragments for tumor-specific targeting of cytotoxic effectors
- Microbial cell-surface display.
- Cancer therapy with engineered monoclonal antibodies
- High-throughput antibody isolation.
- Peptides as drugs: from screening to application.
- A universal strategy for stable intracellular antibodies.
- Cell-Surface display of heterologous proteins: From high-throughput screening to environmental applications.
- Escherichia coli maltose-binding protein as a molecular chaperone for recombinant intracellular cytoplasmic single-chain antibodies.
- Development of a Cell Surface Display System in a Magnetotactic Bacterium, “Magnetospirillum magneticum” AMB-1
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