Selective phage infection mediated by epitope expression on F pilus.
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Summary
In a model library, where a scFv antibody against the human cytomegalovirus AD-2 epitope was selected, an enrichment of 2500 of phage carrying the specific antibody, indicating an efficient selective infection.
- Type
- article
- Published
- 1997-10-31
- Cited by
- 40
- References
- 25
- OpenAlex
- https://openalex.org/W2013505174
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:9252491
Keywords
Pilus, Pilin, Biology, Phagemid, Bacteriophage
References
- Beginning a Genetic Analysis of Conjugational Transfer Determined by the F Factor in Escherichia coli by Isolation and Characterization of Transfer-Deficient Mutants
- Bacterial outer membranes: Biogenesis and functions
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- Structure and function of conjugative pili: inducible synthesis of functional F pili by Escherichia coli K-12 containing a lac-tra operon fusion
- Analysis of the sequence and gene products of the transfer region of the F sex factor
- Structure and function of conjugative pili: monoclonal antibodies as probes for structural variants of F pili
- Examination of the phosphate in conjugative F-like pili by use of electron spectroscopic imaging
- Clonal Selection and Amplification of Phage Displayed Antibodies by Linking Antigen Recognition and Phage Replication
- Co‐selection of cognate antibody‐antigen pairs by selectively‐infective phages
- Structure of F-pili: reassessment of the symmetry.
- Phage antibodies: filamentous phage displaying antibody variable domains
- Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.
- HTLV-III/LAV-neutralizing antibodies to an E. coli-produced fragment of the virus envelope.
- Antibody-selectable filamentous fd phage vectors: affinity purification of target genes.
- The gp116 of the gp58/116 complex of human cytomegalovirus represents the amino-terminal part of the precursor molecule and contains a neutralizing epitope.
- Selection of binders from phage displayed antibody libraries using the BIAcore biosensor.
- Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface.
- Assembly of combinatorial antibody libraries on phage surfaces: the gene III site.
- Light chain shuffling of a high affinity antibody results in a drift in epitope recognition.
Cited by
- The cloning, characterisation and engineering of an IGF-I-BINDING single chain Fv
- Phage display of combinatorial peptide and protein libraries and their applications in biology and chemistry.
- Functional genomics with protein-protein interactions.
- Development of recombinant antibody technology for application in plant pathogen diagnosis
- Evolutionary approaches to protein engineering.
- Pili and flagella biology, structure, and biotechnological applications.
- Fine Affinity Discrimination by Yeast Surface Display and Flow Cytometry
- Comparison of Proteins Involved in Pilus Synthesis and Mating Pair Stabilization from the Related Plasmids F and R100-1: Insights into the Mechanism of Conjugation
- Antibodies from phage antibody libraries.
- Filamentous phage structure, infection and assembly.
- Selective infection of E. coli as a function of a specific molecular interaction
- Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries.
- Design of synthetic antibody libraries
- Generation of recombinant antibodies
- A phage‐based system to select multiple protein‐protein interactions simultaneously from combinatorial libraries
- Antibody Fab display and selection through fusion to the pIX coat protein of filamentous phage.
- Biotechnological applications of phage and cell display.
- Highly efficient selection of phage antibodies mediated by display of antigen as Lpp-OmpA' fusions on live bacteria.
- Recombinant antibody fragments.
- Antibody phage display technology and its applications.
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- Design and use of phage display libraries for the selection of antibodies and enzymes.
- A traC mutant that retains sensitivity to f1 bacteriophage but lacks F pili
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