A Sortase A programmable phage display format for improved panning of Fab antibody libraries
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Summary
A new phagemid display system that addresses the complication of bald phage propagation and improves the selection efficiency of antibody libraries by improving the enrichment of target- reactive mAbs by depleting background phage.
- Type
- article
- Published
- 2018-09-11
- Cited by
- 5
- References
- 57
- Access
- Open access
- OpenAlex
- https://openalex.org/W2891666054
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:52270600
Keywords
Panning (audio), Phage display, Phagemid, Monoclonal antibody, Antibody
References
- Protein production by auto-induction in high density shaking cultures.
- Generation and selection of rabbit antibody libraries by phage display.
- A helper phage to improve single-chain antibody presentation in phage display
- Libraries of peptides and proteins displayed on filamentous phage.
- Sortase Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody Drug Conjugates with High In Vitro and In Vivo Potency
- Isolation of bacteria envelope proteins.
- Engineering M13 for phage display.
- Systematic Characterization and Comparative Analysis of the Rabbit Immunoglobulin Repertoire
- Phage display screening without repetitious selection rounds.
- An improved filamentous helper phage for generating single-stranded plasmid DNA.
- Effects of bacteriophage f1 gene III protein on the host cell membrane
- Phage-display selection on tumor histological specimens with laser capture microdissection
- Noninvasive imaging of immune responses
- Model systems to study the parameters determining the success of phage antibody selections on complex antigens.
- Chimeric rabbit/human Fab and IgG specific for members of the Nogo-66 receptor family selected for species cross-reactivity with an improved phage display vector.
- Phage antibodies: filamentous phage displaying antibody variable domains
- Sortagging: a versatile method for protein labeling.
- A human monoclonal antibody drug and target discovery platform for B-cell chronic lymphocytic leukemia based on allogeneic hematopoietic stem cell transplantation and phage display.
- Filamentous phage infection-mediated gene expression: construction and propagation of the gIII deletion mutant helper phage R408d3.
- Functional analysis of bacteriophage f1 intergenic region.
Cited by
- Phage display antibody libraries: A robust approach for generation of recombinant human monoclonal antibodies.
- Semi-Automated Cell Panning for Efficient Isolation of FGFR3-Targeting Antibody
- Concerted antibody and antigen discovery by differential whole-cell phage display selections and multi-omic target deconvolution
- Employing unnatural promiscuity of sortase to construct peptide macrocycle libraries for ligand discovery
- The Midas touch: Recombinant antibodies transforming diagnostic platforms.
- Keystone Symposium Antibodies as Drugs 2019 – New Horizons in the Therapeutic Use of Engineered Antibodies
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