Global analysis of protein expression in yeast
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Summary
A Saccharomyces cerevisiae fusion library is created where each open reading frame is tagged with a high-affinity epitope and expressed from its natural chromosomal location, and it is found that about 80% of the proteome is expressed during normal growth conditions.
- Type
- article
- Published
- 2003-10-16
- Cited by
- 3,974
- References
- 31
- OpenAlex
- https://openalex.org/W2013947447
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:4344864
Keywords
Proteome, Saccharomyces cerevisiae, Biology, Computational biology, Messenger RNA
References
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- Biology's new Rosetta stone
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- Finding Functional Features in Saccharomyces Genomes by Phylogenetic Footprinting
- Four years of post‐genomic life with 6000 yeast genes
- The B-type cyclin kinase inhibitor p40SIC1 controls the G1 to S transition in S. cerevisiae.
- Total number of coding open reading frames in the yeast genome
- Iterative signature algorithm for the analysis of large-scale gene expression data.
- Functional organization of the yeast proteome by systematic analysis of protein complexes
- Protein pathway and complex clustering of correlated mRNA and protein expression analyses in Saccharomyces cerevisiae
- Differential function and expression of Saccharomyces cerevisiae B-type cyclins in mitosis and meiosis
- Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization.
Cited by
- Advanced Techniques in Mass Spectrometry for Qualitative and Quantitative Protein Characterization
- Systems Level Modeling of the Cell Cycle Using Budding Yeast
- Regulation of mitotic progression in Saccharomyces cerevisiae by the microtubule-associated proteins Slk19 and Stu1
- Advances in pharmacogenomics and individualized drug therapy: exciting challenges that lie ahead.
- Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits.
- Defining the SUMO-modified Proteome by Multiple Approaches in Saccharomyces cerevisiae*
- Nop53p is required for late 60S ribosome subunit maturation and nuclear export in yeast.
- Protein function, connectivity, and duplicability in yeast.
- Nuclear Import of Ho Endonuclease Utilizes Two Nuclear Localization Signals and Four Importins of the Ribosomal Import System*
- Contribution of Trf4/5 and the Nuclear Exosome to Genome Stability Through Regulation of Histone mRNA Levels in Saccharomyces cerevisiae
- Analysis of protein-protein interactions in mitochondria.
- Utp8p is a nucleolar tRNA-binding protein that forms a complex with components of the nuclear tRNA export machinery in Saccharomyces cerevisiae.
- A biological interpretation of transient anomalous subdiffusion. II. Reaction kinetics.
- Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis.
- Revisiting the Saccharomyces cerevisiae predicted ORFeome.
- Summary of United States Human Proteome Organisation (HUPO) Symposium Entitled “Standardized Clinical Proteomics Platforms”
- Analysis of iron–sulfur protein maturation in eukaryotes
- Thioredoxin-related protein 14, a new member of the thioredoxin family with disulfide reductase activity: implication in the redox regulation of TNF-alpha signaling.
- The Interactorium: Visualising proteins, complexes and interaction networks in a virtual 3‐D cell
- A computational approach to inferring cellular protein‐binding affinities from quantitative fluorescence resonance energy transfer imaging
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