Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer.
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Summary
An assessment of the practical value to the clinician of RT-PCR-based molecular diagnostics concludes that there is an urgent need for standardisation at every level, starting with pre-asset sample acquisition and template preparation, assay protocols and post-assay analysis.
- Type
- review
- Published
- 2006-04-01
- Cited by
- 46
- References
- 179
- OpenAlex
- https://openalex.org/W1975555474
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:2162137
Keywords
Colorectal cancer, Disease, Medicine, Oncology, Lymph node
References
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Cited by
- Cellular and molecular targets of silibinin, a natural flavonoid, in colorectal cancer prevention and therapy
- Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
- Prognostic Value of Molecular Detection of Lymph Node Metastases After Curative Resection of Stage II Colon Cancer: A Systematic Pooled Data Analysis.
- RNA isolation and quantitative PCR from HOPE- and formalin-fixed bovine lymph node tissues.
- Quantification of mRNA using real-time RT-PCR
- Evaluation of Alpha 1-Antitrypsin and the Levels of mRNA Expression of Matrix Metalloproteinase 7, Urokinase Type Plasminogen Activator Receptor and COX-2 for the Diagnosis of Colorectal Cancer
- Evaluation of poly(ethylene glycol)-coated monodispersed magnetic poly(2-hydroxyethyl methacrylate) and poly(glycidyl methacrylate) microspheres by PCR
- Molecular medicine, gene-expression profiling and molecular diagnostics: putting the cart before the horse.
- Evaluation of Guanylyl Cyclase C Lymph Node Status for Colon Cancer Staging and Prognosis
- Nanocolonies and diagnostics of oncological diseases associated with chromosomal translocations
- Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients
- Circulating tumour cells in colorectal cancer
- Evaluation of the Adenocarcinoma-Associated Gene AGR2 and the Intestinal Stem Cell Marker LGR5 as Biomarkers in Colorectal Cancer
- Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?
- Predictors of outcome in an AIEOP series of childhood ependymomas: a multifactorial analysis.
- Cytokine gene and protein expression in BCG vaccinated and non-vaccinated Mycobacterium bovis infected cattle
- Molecular staging of colorectal cancer: new paradigm or waste of time?
- Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer
- RNA biomarkers in colorectal cancer.
- Nucleic acid quantification and disease outcome prediction in colorectal cancer.
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