Delayed protein shut down and cytopathic changes lead to high yields of infectious pancreatic necrosis virus cultured in Asian Grouper cells.
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Summary
It is suggested that high cell numbers and prolonged survival of AGK cells are responsible for the superior virus yields over RTG-2 and higher split ratio/shorter split interval makes AGK superior over CHSE cells.
- Type
- article
- Published
- 2014-01-01
- Cited by
- 10
- References
- 32
- OpenAlex
- https://openalex.org/W24422212
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:24281963
Keywords
Medicine, Vascular access, Cardiology, Intensive care medicine, Internal medicine
References
- Infectious Pancreatic Necrosis Virus VP5 Is Dispensable for Virulence and Persistence
- Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: A putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif
- Development of an in vitro system to measure the sensitivity to the antiviral Mx protein of fish viruses.
- Size and structure of the genome of infectious pancreatic necrosis virus.
- Quantification of mRNA using real-time RT-PCR
- Apoptosis precedes necrosis of fish cell line with infectious pancreatic necrosis virus infection.
- Immunogenicity and Cross Protective Ability of the Central VP2 Amino Acids of Infectious Pancreatic Necrosis Virus in Atlantic Salmon (Salmo salar L.)
- The nucleotide sequence of infectious pancreatic necrosis virus (IPNV) dsRNA segment A reveals one large ORF encoding a precursor polyprotein.
- A protein that replaces the entire cellular eIF4F complex
- Frequent occurrence of apoptosis is not associated with pathogenic infectious pancreatic necrosis virus (IPNV) during persistent infection.
- Fish cell lines: Establishment and characterization of nine cell lines from salmonids
- Comparison of vaccine efficacy for different antigen delivery systems for infectious pancreatic necrosis virus vaccines in Atlantic salmon (Salmo salar L.) in a cohabitation challenge model.
- EFFECTS OF TEMPERATURE ON GROWTH RATE OF CULTURED MAMMALIAN CELLS (L5178Y)
- The interplay between infectious pancreatic necrosis virus (IPNV) and the IFN system: IFN signaling is inhibited by IPNV infection
- Differentially expressed genes following persistent infection with infectious pancreatic necrosis virus in vitro and in vivo.
- Physico-chemical and morphological features of infectious pancreatic necrosis virus.
- The kinetics of CD4+ and CD8+ T-cell gene expression correlate with protection in Atlantic salmon (Salmo salar L) vaccinated against infectious pancreatic necrosis.
- Infectious pancreatic necrosis virus persistently infects chinook salmon embryo cells independent of interferon.
- An antiviral state induced in Chinook salmon embryo cells (CHSE-214) by transfection with the double-stranded RNA poly I:C.
- Global protein synthesis shutdown in Autographa californica nucleopolyhedrovirus-infected Ld652Y cells is rescued by tRNA from uninfected cells.
Cited by
- Infection Profiles of Selected Aquabirnavirus Isolates in CHSE Cells
- Oral Vaccination of Fish – Antigen Preparations, Uptake, and Immune Induction
- A Piscine Birnavirus Induces Inhibition of Protein Synthesis in CHSE-214 Cells Primarily through the Induction of eIF2α Phosphorylation
- In vitro adaptation of SAV3 in cell culture correlates with reduced in vivo replication capacity and virulence to Atlantic salmon (Salmo salar L.) parr.
- Oral delivery of protein antigens to Atlantic salmon
- Study of RNA-A Initiation Translation of The Infectious Pancreatic Necrosis Virus.
- Apoptosis Induction by dsRNA-Dependent Protein Kinase R (PKR) in EPC Cells via Caspase 8 and 9 Pathways
- Development and characterization of megalocytivirus persistently-infected cell cultures for high yield of virus.
- The RNA-dependent RNA polymerase of the infectious pancreatic necrosis virus is linked to viral mRNA acting as a cap substitute.
- In vitro adaptation of SAV 3 in cell culture correlates with reduced in vivo replication capacity and virulence to Atlantic salmon ( Salmo salar L . )
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