PFGE and YAC analysis of the Arabidopsis genome
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Summary
PFGE and blot hybridization analyses of Arabidopsis DNA-containing yeast artificial chromosomes demonstrated that both unique and highly repeated DNA sequences are represented.
- Type
- article
- Published
- 1990-10-01
- Cited by
- 81
- References
- 32
- Access
- Open access
- OpenAlex
- https://openalex.org/W1990578762
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:83485241
Keywords
Arabidopsis, Yeast artificial chromosome, Biology, Cloning (programming), Molecular cloning
References
- Identification of the yeast DNA sequences that correspond to specific tyrosine-inserting nonsense suppressor loci.
- Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.
- Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome
- An electrophoretic karyotype of Neurospora crassa
- Construction of a genetic linkage map in man using restriction fragment length polymorphisms.
- Cloning of human telomeres by complementation in yeast
- Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster.
- Separation of large DNA molecules by contour-clamped homogeneous electric fields.
- An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.
- A 12 megabase restriction map at the cystic fibrosis locus.
- Genome linking with yeast artificial chromosomes
- A common language for physical mapping of the human genome.
- Transformation of yeast spheroplasts without cell fusion.
- Mapping the Drosophila genome with yeast artificial chromosomes.
- Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
- Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.
- Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.
- Isolation and characterization of a human telomere
- Isolation of single-copy human genes from a library of yeast artificial chromosome clones.
Cited by
- Physical Map and Organization of Arabidopsis thaliana Chromosome 4
- High molecular weight DNA extraction from Arabidopsis.
- Protection of megabase-sized chromosomal DNA from breakage by DNase activity in plant nuclei.
- Biochemical characterization and positional cloning of the var2 variegation mutant of Arabidopsis thaliana
- Chromosome walking with YAC clones in Arabidopsis: isolation of 1700 kb of contiguous DNA on chromosome 5, including a 300 kb region containing the flowering-time gene CO
- Oryza: From Molecule to Plant
- Physical mapping of the rice genome with BACs
- Genome mapping, molecular markers and marker-assisted selection in crop plants
- Localization of AtROP4 and AtROP6 and interaction with the guanine nucleotide dissociation inhibitor AtRhoGDI1 from Arabidopsis
- Members of the Arabidopsis actin gene family are widely dispersed in the genome.
- Mapping expressed sequence tag sites on yeast artificial chromosome clones of Arabidopsis thaliana DNA.
- Advantages of Arabidopsis for cloning plant genes
- Analysis of clones carrying repeated DNA sequences in two YAC libraries of Arabidopsis thaliana DNA.
- The Arabidopsis thaliana genome has multiple divergent forms of phosphoinositol-specific phospholipase C1.
- Construction of a YAC library from a Beta vulgaris fragment addition and isolation of a major satellite DNA cluster linked to the beet cyst nematode resistance locus Hs1pat−1
- Arabidopsis YAC restriction mapping.
- Detailed description of four YAC contigs representing 17 Mb of chromosome 4 of Arabidopsis thaliana ecotype Columbia.
- Analysis of Underivatized Brassinosteroids by HPLC/APCI-MS. Occurrence of 3-Epibrassinolide in Arabidopsis thaliana
- Isolation of an 800 kb contiguous DNA fragment encompassing a 3.5-cM region of chromosome 1 in Arabidopsis using YAC clones.
- CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases.
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