A Processive Protein Chimera Introduces Mutations Across Defined DNA Regions In Vivo
Explore this paper's citation graph
Summary
The development and application of the MutaT7 chimera is reported, a potent and highly targeted in vivo mutagenesis agent that utilizes a DNA-damaging cytidine deaminase fused to a processive RNA polymerase to continuously direct mutations to specific, well-defined DNA regions of any relevant length.
- Type
- article
- Published
- 2018-07-10
- Cited by
- 129
- References
- 37
- Access
- Open access
- OpenAlex
- https://openalex.org/W2836805751
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:49741325
Keywords
Mutagenesis, DNA, Computational biology, Chimera (genetics), Cytidine deaminase
References
- A mutation in the folA promoter delays adaptation to minimal medium by Escherichia coli K‐12
- Promoter specificity determinants of T7 RNA polymerase.
- An orthogonal DNA replication system in yeast.
- A mutant T7 phage promoter is specifically transcribed by T7-RNA polymerase in mammalian cells.
- A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions.
- Genetic aspects and epidemiologic implications of resistance to trimethoprim.
- Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I
- Protein p56 from the Bacillus subtilis phage ϕ29 inhibits DNA-binding ability of uracil-DNA glycosylase
- Yeast Oligo-mediated Genome Engineering (YOGE)
- Mutagenic Effects of Hydroxylamine in vivo
- Uracil-DNA glycosylase (UNG)-deficient mice reveal a primary role of the enzyme during DNA replication.
- Transcription enhances AID-mediated cytidine deamination by exposing single-stranded DNA on the nontemplate strand
- An efficient random mutagenesis technique using an E. coli mutator strain.
- Evolutionary origins of apoB mRNA editing: catalysis by a cytidine deaminase that has acquired a novel RNA-binding motif at its active site.
- Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase.
- Biological Costs and Mechanisms of Fosfomycin Resistance in Escherichia coli
- Roles of DNA polymerase I in leading and lagging-strand replication defined by a high-resolution mutation footprint of ColE1 plasmid replication
- Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus.
- T7 RNA polymerase-directed transcripts are processed in yeast and link 3' end formation to mRNA nuclear export.
- Use of the rpoB gene to determine the specificity of base substitution mutations on the Escherichia coli chromosome.
Cited by
- Scalable, continuous evolution of genes at mutation rates above genomic error thresholds
- An Adaptable Platform for Directed Evolution in Human Cells
- Probing pathways of adaptation with continuous evolution
- In vivo Protein Evolution, Next Generation Protein Engineering Strategy: from Random Approach to Target-specific Approach
- Engineering polymerases for new functions
- Pseudomonas putida in the quest of programmable chemistry.
- Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotype
- Tools and systems for evolutionary engineering of biomolecules and microorganisms
- Genetic Engineering by DNA Recombineering
- In vivo diversification of target genomic sites using processive T7 RNA polymerase-base deaminase fusions blocked by RNA-guided dCas9
- A Phage-Assisted Continuous Selection Approach for Deep Mutational Scanning of Protein-Protein Interactions.
- Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor
- Chemical Biology Framework to Illuminate Proteostasis
- Automated continuous evolution of proteins in vivo
- The developing toolkit of continuous directed evolution
- Current Status and Applications of Adaptive Laboratory Evolution in Industrial Microorganisms
- Combining protein and metabolic engineering to construct efficient microbial cell factories.
- In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
- Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo
- Full-Spectrum Targeted Mutagenesis in Plant and Animal Cells
Related papers
- An Enzymatic Assay for High-Throughput Screening of Cytidine-Producing Microbial Strains
- Regulation of pyrimidine nucleotide biosynthesis in cytidine deaminase-negative mutants of Bacillus subtilis
- Cytidine deaminase: a rapid method of purification and some properties of the enzyme from human placenta.
- A Quantum Chemical Study of the Catalysis for Cytidine Deaminase: Contribution of the Extra Water Molecule
- Knockout of the cdd Gene in Bacillus subtilis and its Influence on Cytidine Fermentation
- Biochemical and structural analysis of the Klebsiella pneumoniae cytidine deaminase CDA.