High level gene expression in mammalian cells by a nuclear T7-phase RNA polymerase.
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Summary
A novel expression system for mammalian cells which is based on transcription of hybrid genes containing T7 phage promoters by a T7phage RNA polymerase targeted to the nucleus of the host cells is described.
- Type
- article
- Published
- 1989-11-11
- Cited by
- 53
- References
- 1
- Access
- Open access
- OpenAlex
- https://openalex.org/W2013673762
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:8157557
Keywords
Biology, T7 RNA polymerase, Gene, Polymerase, Nucleic acid
References
No references recorded for this paper.
Cited by
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- The Immunogenic Epitopes of the Glycoproteins of Human Metapneumovirus
- Stable high-level gene expression in mammalian cells by T7 phage RNA polymerase.
- Construction of an eGFP Expression Plasmid under Control of T7 Promoter and IRES Sequence for Assay of T7 RNA Polymerase Activity in Mammalian Cell Lines
- Selection of efficient cleavage sites in target RNAs by using a ribozyme expression library
- Functional roles for the TATA promoter and enhancers in basal and Tat-induced expression of the human immunodeficiency virus type 1 long terminal repeat
- Structural analysis of vaccinia virus DIs strain: application as a new replication-deficient viral vector.
- A Hybrid Baculovirus-Bacteriophage T7 Transient Expression System
- A T7 promoter-specific, inducible protein expression system forBacillus subtilis
- A mutant T7 phage promoter is specifically transcribed by T7-RNA polymerase in mammalian cells.
- Expression of biologically active HIV glycoproteins using a T7 RNA polymerase-based eucaryotic vector system
- Vaccinia virus: a tool for research and vaccine development
- A hybrid baculovirus-T7 RNA polymerase system for recovery of an infectious virus from cDNA.
- T7 RNA polymerase drives transcription of a reporter gene from T7 promoter, but engenders post-transcriptional silencing of expression
- New mammalian expression vectors
- shRNA driven by Pol II/T7 dual-promoter system effectively induce cell-specific RNA interference in mammalian cells.
- A phage T7 class-III promoter functions as a polymerase II promoter in mammalian cells.
- Gene expression using DNA viral vectors.
- The C-terminal domain of RNA polymerase II couples mRNA processing to transcription
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