Immobilized pH gradients
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Summary
An account of the steps through which the protocols for operation with IPGs were set up in the early '80s, and the development of a computer program for the calculation of pH, buffering power and ionic strength of a mixture of monoprotic buffers titrated within any pH span and for the linearization of the desired pH gradient.
- Type
- review
- Published
- 2009-06-01
- Cited by
- 358
- References
- 58
- OpenAlex
- https://openalex.org/W1988886084
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:32142824
Keywords
Computer science, Immobilized pH gradient, Capillary electrophoresis, Chemistry, Chromatography
References
- Immobilized Ph Gradients: Theory and Methodology
- Isoelectric focusing in layers of granulated gels. I. Thin-layer isoelectric focusing of proteins.
- Pouring reproducible gradients in gels under computer control: new devices for simultaneous delivery of two independent gradients, for more flexible slope and pH range of immobilized pH gradients.
- Electrophoretically silent hemoglobin mutants as revealed by isoelectric focusing in immobilized pH gradients
- Urine analysis by two‐dimensional gel eletrophoresis with isoelectric focusing in immobilized pH gradients in the first dimension
- Isoelectric focusing in layers of granulated gels. II. Preparative isoelectric focusing.
- Synthesis of thiomorpholino buffers for isoelectric focusing in immobilized pH gradients
- Polymerization kinetics of polyacrylamide gels containing immobilized pH gradients for isoelectric focusing
- Non‐linear pH courses with immobilized pH gradients
- Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications.
- Serum protein analysis on immobilized pH gradients with in situ adsorption of albumin on Dextran Blue
- Isoelectric focusing of basic proteases in immobilized pH gradients.
- Electrotransfer of proteins after isoelectric focusing (with or without urea) on fabric-reinforced, immobilized pH gradient gels.
- Two‐dimensional analysis of membrane proteins with isoelectric focusing in immobilized pH gradients in the first dimension
- Two-dimensional maps in very acidic immobilized pH gradients.
- Buffer systems and pH gradient simulation
- Isoelectric focusing in immobilized pH gradients: generation and optimization of wide pH intervals with two-chamber mixers.
- Immobilized pH gradients: Effect of salts, added carrier ampholytes and voltage gradients on protein patterns
- Isoelectric focusing in immobilized pH gradients: generation of extended pH intervals.
- Structure-stability relationship of immobiline chemicals for isoelectric focusing as monitored by capillary zone electrophoresis
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