A novel synthesis and detection method for cap-associated adenosine modifications in mouse mRNA
Explore this paper's citation graph
Summary
A novel method for the synthesis of modified adenosine phosphoramidites was developed and the product can directly be used for the production of N6,2'-O-dimethyladenosine containing RNA oligonucleotides.
- Type
- article
- Published
- 2011-10-24
- Cited by
- 63
- References
- 45
- Access
- Open access
- OpenAlex
- https://openalex.org/W1977233304
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10848695
Keywords
Guanosine, Nucleotide, Oligonucleotide, Messenger RNA, Gene
References
- Simplified sample preparation for the analysis of oligonucleotides by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry.
- HeLa cell RNA (2'-O-methyladenosine-N6-)-methyltransferase specific for the capped 5'-end of messenger RNA.
- in maize poly(A)-containing RNA
- The characterization of growth factor activity in human brain.
- Post-transcriptional modifications of mRNA. Purification and characterization of cap I and cap II RNA (nucleoside-2'-)-methyltransferases from HeLa cells.
- Purines—VI: Rate study and mechanism of the dimroth rearrangement of 1-alkoxy-9-alkyladenines and 1-alkyl-9-methyladenines
- 5'-terminal cap structure in eucaryotic messenger ribonucleic acids.
- Purification and cDNA cloning of the AdoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase.
- Precise localization of m6A in Rous sarcoma virus RNA reveals clustering of methylation sites: implications for RNA processing
- Nucleotides adjacent to N6-methyladenosine in maize poly(A)-containing RNA
- Induction of sporulation in Saccharomyces cerevisiae leads to the formation of N6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4 gene.
- Biophysical studies of eIF4E cap-binding protein: recognition of mRNA 5' cap structure and synthetic fragments of eIF4G and 4E-BP1 proteins.
- N6, O2′-dimethyladenosine a novel methylated ribonucleoside next to the 5′ terminal of animal cell and virus mRNAs
- Influence of 5'-terminal m7G and 2'--O-methylated residues on messenger ribonucleic acid binding to ribosomes.
- Alkylation of 6-N-acylated adenosine derivatives by the use of phase transfer catalysis
- The Synthesis of 2′-O-[(Triisopropylsilyl)oxy] methyl (TOM) Phosphoramidites of Methylated Ribonucleosides (m1G, m2G, m22G, m1I, m3U, m4C, m6A, m62A) for Use in Automated RNA Solid-Phase Synthesis
- 2′-O-ribose methylation of cap2 in human: function and evolution in a horizontally mobile family
- Crystal structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound human full-length eukaryotic initiation factor 4E: biological importance of the C-terminal flexible region.
- Purines. XXIX: Syntheses of 9-alkyl-2-deuterio-N6-methoxyadenines and 2-deuterio-N6,9-dimethyladenine: tautomerism in 9-substituted N6-alkoxyadenines
- In Vitro Reconstitution of SARS-Coronavirus mRNA Cap Methylation
Cited by
- The Arabidopsis epitranscriptome.
- Mapping and significance of the mRNA methylome
- Yeast m6A Methylated mRNAs Are Enriched on Translating Ribosomes during Meiosis, and under Rapamycin Treatment
- Investigating the enzymology and molecular function of 5-methylctosine in RNA
- m6A level and isoform characterization sequencing (m6A-LAIC-seq) reveals the census and complexity of the m6A epitranscriptome
- Reversible methylation of m6Am in the 5′ cap controls mRNA stability
- The N6-methyladenosine (m6A)-forming enzyme METTL3 controls myeloid differentiation of normal and leukemia cells
- Regulation and function of CMTR1‐dependent mRNA cap methylation
- Distinguishing RNA modifications from noise in epitranscriptome maps.
- FTO, m6Am, and the hypothesis of reversible epitranscriptomic mRNA modifications
- The RNA demethylase FTO targets m6Am in snRNA to establish distinct methyl isoforms that influence splicing
- mRNA cap regulation in mammalian cell function and fate
- PCIF1 catalyzes m6Am mRNA methylation to regulate gene expression
- CAPAM: The mRNA Cap Adenosine N6-Methyltransferase
- FTO controls reversible m6Am RNA methylation during snRNA biogenesis
- Combining chemical synthesis and enzymatic methylation to access short RNAs with various 5′ caps
- Detection and analysis of RNA methylation
- Antibody cross-reactivity accounts for widespread appearance of m1A in 5’UTRs
- DHX15 regulates CMTR1-dependent gene expression and cell proliferation
- Diversity and abundance model according to habitat characteristics of filariasis vector, Mansonia spp. in Banyuasin, South Sumatera, Indonesia
Related papers
- Effects of adenosine on levels of adenosine cyclic 3',5'-monophosphate in human blood platelets in relation to adenosine incorporation and platelet aggregation.
- Reactive and exercise hyperemia during high levels of adenosine infusion.
- Myocardial protection: The adenosine story
- Adenosine and adenosine analogues increase blood flow in oral mucosa.
- Elevation of guanosine 3',5'-monophosphate level by adenosine in cerebellar slices of guinea pig.
- Abstract 524: Extracellular Guanosine Regulates Extracellular Adenosine Levels
- Complementary addressed modification of oligonucleotide d(pGpGpCpGpGpA) with platinum derivative of oligonucleotide d(pTpCpCpGpCpCpTpTpT)
- Kinetics of interaction of nucleotides with nucleotide-free H-ras p21.
- Spin-labelled analogues of GDP and GTP as site-specific reporter groups for guanosine nucleotide-binding proteins.
- Association of alteration of nucleosides and nucleotides with gastric cancer microenvironment