Bacterial Expression, Facile Purification and Properties of Recombinant Human Lymphotoxin (Tumor Necrosis Factor Beta)
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Summary
Cell lines varied greatly in their sensitivity to rhTNF–β and a different pattern of response was seen in the cytostatic and cytotoxic assays, indicating that some but not all cells require uninterrupted protein synthesis in order to protect themselves from TNF-β cytotoxicity.
- Type
- article
- Published
- 1989-04-01
- Cited by
- 23
- References
- 37
- OpenAlex
- https://openalex.org/W1970693659
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:23121858
Keywords
Lymphotoxin, Cycloheximide, Cytotoxic T cell, Cell culture, Tumor necrosis factor alpha
References
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- Human tumor necrosis factor. Production, purification, and characterization.
- Tumor necrosis factor induces synthesis of two proteins in human fibroblasts.
- Human lymphotoxin. Production by a lymphoblastoid cell line, purification, and initial characterization.
- Recombinant human TNF induces production of granulocyte–monocyte colony-stimulating factor
- Site-specific mutagenesis of the human fibroblast interferon gene.
- Molecular cloning of the complementary DNA for human tumor necrosis factor.
- Expression plasmid vectors containing Escherichia coli tryptophan promoter transcriptional units lacking the attenuator.
- Passive immunization against cachectin/tumor necrosis factor protects mice from lethal effect of endotoxin.
- A role for mRNA secondary structure in the control of translation initiation
- Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro.
- Purification of cachectin, a lipoprotein lipase-suppressing hormone secreted by endotoxin-induced RAW 264.7 cells
- Cloning and expression of cDNA for human lymphotoxin, a lymphokine with tumour necrosis activity
- Cloning and polypeptide analysis of the leading region in F plasmid DNA transfer.
- Induction of tumor necrosis factor expression and resistance in a human breast tumor cell line.
- Formation of Soluble Recombinant Proteins in Escherichia Coli is Favored by Lower Growth Temperature
- HLA class II induction in human islet cells by interferon-γ plus tumour necrosis factor or lymphotoxin
- Induction and regulation of mRNA encoding 26-kDa protein in human cell lines treated with recombinant human tumor necrosis factor.
- Secondary structure of mRNA and efficiency of translation initiation.
Cited by
- The ligands and receptors of the lymphotoxin system.
- Investigation of biosynthesis of human lymphotoxin in cells of recombinant Escherichia coli strain
- Two discrete types of tumor necrosis factor-resistant cells derived from the same cell line.
- Solubility and secretability.
- Role of polyethyleneimine in the purification of recombinant human tumour necrosis factor beta.
- A Simple genetically structured model of trp repressor–operator interactions
- Antibody against branched epitope as an affinity ligand to separate the parent protein.
- Effect of local secondary structure of mRNA on expression of recombinant human lymphotoxin inEscherichia coli
- Formulation and optimization of two culture media for the production of tumour necrosis factor-β in Escherichia coli
- Purification and renaturation of recombinant human lymphotoxin (tumour necrosis factor beta) expressed in Escherichia coli as inclusion bodies.
- Preparation and Characterization of Soluble Recombinant Heterotrimeric Complexes of Human Lymphotoxins and (*)
- Flow-injection analysis : wall-jet electrode system for monitoring glucose and lactate in fermentation broths
- Tumor necrosis factor Characterization at the molecular, cellular and in vivo level
- A rapid procedure for production of human basic fibroblast growth factor in Escherichia coli cells.
- Genetically structured mathematical modeling of trp attenuator mechanism.
- Recombinant human tumor necrosis factor-beta entrapped in liposomes formed by a modification of the dehydration-rehydration method retains potent cytotoxic activity on L929 cells in vitro.
- Functional discrepancies between tumor necrosis factor and lymphotoxin α explained by trimer stability and distinct receptor interactions
- Gene expression using gram-negative bacteria.
- Induction of inflammatory cell infiltration and necrosis in normal mouse skin by the combined treatment of tumor necrosis factor and lithium chloride.
- Growth and Induction Effects on the Expression of rhTNF-ß
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