Mechanism of CDK activation revealed by the structure of a cyclinA-CDK2 complex
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Summary
The crystal structure of the human cyclinA-cyclin-dependent kinase2-ATP complex has been determined at 2.3 A resolution and activates the kinase by realigning active site residues and relieving the steric blockade at the entrance of the catalytic cleft.
- Type
- article
- Published
- 1995-07-27
- Cited by
- 1,451
- References
- 46
- OpenAlex
- https://openalex.org/W1969939692
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:4361179
Keywords
Cyclin-dependent kinase, Cyclin-dependent kinase 2, Steric effects, Chemistry, Kinase
References
- Differential regulation of E2F transactivation by cyclin/cdk2 complexes.
- The cell cycle kinases.
- A‐ and B‐type cyclins differentially modulate substrate specificity of cyclin‐cdk complexes.
- Experiences with a new translation-function program
- A family of cyclin homologs that control the G1 phase in yeast.
- Targets of cyclin-dependent protein kinases.
- Atomic structure of the MAP kinase ERK2 at 2.3 Å resolution
- Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals.
- The interpretation of protein structures: estimation of static accessibility.
- Dictionary of protein secondary structure: Pattern recognition of hydrogen‐bonded and geometrical features
- Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.
- Functional interactions of the retinoblastoma protein with mammalian D-type cyclins.
- Three protein kinase structures define a common motif.
- Improved methods for building protein models in electron density maps and the location of errors in these models.
- MERLOT, an integrated package of computer programs for the determination of crystal structures by molecular replacement
- Insights into autoregulation from the crystal structure of twitchin kinase
- Maternal mRNA from clam oocytes can be specifically unmasked in vitro by antisense RNA complementary to the 3'-untranslated region.
- Activation of the various cyclin/cdc2 protein kinases.
- Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast.
- A novel cyclin associates with M015/CDK7 to form the CDK-activating kinase
Cited by
- Development of an shRNA screen to identify mediators of cellular senescence in human breast epithelial cells
- Methods for preparation of proteins and protein complexes that regulate the eukaryotic cell cycle for structural studies.
- Saccharomyces cerevisiae G1 cyclins differ in their intrinsic functional specificities
- Structure of the retinoblastoma tumour-suppressor pocket domain bound to a peptide from HPV E7
- Signal transduction through MAP kinase cascades.
- Human and yeast cdk-activating kinases (CAKs) display distinct substrate specificities.
- Differential expression of genes for cyclin-dependent protein kinases in rice plants.
- Crystal structure of a viral cyclin, a positive regulator of cyclin-dependent kinase 6.
- Tat transactivation: a model for the regulation of eukaryotic transcriptional elongation.
- Conditional transformation of rat embryo fibroblast cells by a cyclin D1-cdk4 fusion gene
- The plant cell cycle.
- Cyclin H activation and drug susceptibility of the Pfmrk cyclin dependent protein kinase from Plasmodium falciparum.
- The mitotic serine/threonine kinase Aurora2/AIK is regulated by phosphorylation and degradation
- Protein-Protein Interactions in Receptor Activation and Intracellular Signalling
- Molecular characterization of Arabidopsis PHO80-like proteins, a novel class of CDKA;1-interacting cyclins
- Novel Functions of the Phospholipase D2-Phox Homology Domain in Protein Kinase Cζ Activation
- CDK activation by non-cyclin proteins.
- A monkey wrench in the kinase machine
- Phospho-p70S6K and cdc2/cdk1 as therapeutic targets for diffuse large B-cell lymphoma
- The protein kinase activity modulation sites: mechanisms for cellular regulation - targets for therapeutic intervention.
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