The Influence of Frame Alignment with Dose Compensation on the Quality of Single Particle Reconstructions
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Summary
It is shown that rigorous dose compensation allows for better particle classifiability and greater recovery of structural information from negatively charged, electron-sensitive side-chains, resulting in a more accurate macromolecular model.
- Type
- article
- Published
- 2015-09-25
- Cited by
- 32
- References
- 42
- Access
- Open access
- OpenAlex
- https://openalex.org/W1781789881
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:31546576
Keywords
Detector, Resolution (logic), Single particle analysis, Cryo-electron microscopy, Electron
References
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- Structural Heterogeneity in Pre-40S Ribosomes
- The 2.8 Å Electron Microscopy Structure of Adeno-Associated Virus-DJ Bound by a Heparinoid Pentasaccharide
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- Map challenge: Analysis using a pair comparison method based on Fourier shell correlation.
- Advances in image processing for single-particle analysis by electron cryomicroscopy and challenges ahead.
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- Molecular biology and structure of a novel penaeid shrimp densovirus elucidate convergent parvoviral host capsid evolution
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