Thon rings from amorphous ice and implications of beam-induced Brownian motion in single particle electron cryo-microscopy

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Summary

Using the measured variation in the power spectra amplitude with number of electrons per image it is deduced that water molecules are randomly displaced by a mean squared distance of ∼1.1 ℬ2 for every incident 300 keV e−/Å2.

Type
article
Published
2015-04-03
Cited by
67
References
19
Access
Open access

Keywords

Brownian motion, Electron microscope, Amorphous solid, Particle (ecology), Cryo-electron microscopy

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