Flexi Vector Cloning
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Summary
A protocol for ligation-dependent cloning using the Flexi Vector method in a 96-well format is described, which includes PCR amplification of the desired gene to append Flexi Vector cloning sequences and transfer of the sequence-verified clones into another Flexi Vector plasmid backbone.
- Type
- article
- Published
- 2009-01-01
- Cited by
- 28
- References
- 22
- Access
- Open access
- OpenAlex
- https://openalex.org/W77472735
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:3279668
Keywords
Cloning (programming), Cloning vector, clone (Java method), Vector (molecular biology), Ligation
References
- High-throughput cloning for proteomics research.
- Emerging bacterial enzyme targets.
- Structural genomics in North America
- Genomics of host-pathogen interactions.
- Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
- High-throughput Purification and Quality Assurance of Arabidopsis thaliana Proteins for Eukaryotic Structural Genomics
- Enzyme Free Cloning for high throughput gene cloning and expression
- Clinical proteomics: new trends for protein microarrays.
- High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system.
- BIOLOGICAL CHARACTERISTICS OF PALINDROMIC DNA(II)
- Overview of structural genomics: from structure to function.
- Coal Poised for a Comeback?
- Results from high-throughput DNA cloning of Arabidopsis thaliana target genes using site-specific recombination
- Multiplexed expression and screening for recombinant protein production in mammalian cells
- Environmental Genome Shotgun Sequencing of the Sargasso Sea
- The Impact of Structural Genomics: Expectations and Outcomes
- Implementation of semi-automated cloning and prokaryotic expression screening: the impact of SPINE.
- A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site.
- The P1' specificity of tobacco etch virus protease.
- Structural genomics
Cited by
- Structure‐guided analysis of catalytic specificity of the abundantly secreted chitosanase SACTE_5457 from Streptomyces sp. SirexAA‐E
- Expression Platforms for Producing Eukaryotic Proteins: A Comparison of E. coli Cell-Based and Wheat Germ Cell-Free Synthesis, Affinity and Solubility Tags, and Cloning Strategies
- Proteins interacting with cloning scars: a source of false positive protein-protein interactions
- IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation
- Expression, purification and characterization of a functional carbohydrate-binding module from Streptomyces sp. SirexAA-E.
- Structural and functional characterization of CalS11, a TDP-rhamnose 3'-O-methyltransferase involved in calicheamicin biosynthesis.
- Biochemical Properties and Atomic Resolution Structure of a Proteolytically Processed β-Mannanase from Cellulolytic Streptomyces sp. SirexAA-E
- Tryptophan-47 in the active site of Methylophaga sp. strain SK1 flavin-monooxygenase is important for hydride transfer.
- Active Site and Laminarin Binding in Glycoside Hydrolase Family 55*
- Recombinant expression, purification, and characterization of ThmD, the oxidoreductase component of tetrahydrofuran monooxygenase.
- High throughput protein production
- Crystal structure of an eIF4G-like protein from Danio rerio
- Structure of T4moF, the Toluene 4-Monooxygenase Ferredoxin Oxidoreductase.
- Use of Nanostructure-Initiator Mass Spectrometry to Deduce Selectivity of Reaction in Glycoside Hydrolases
- Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling
- Relationship between recombinant protein expression and host metabolome as determined by two-dimensional NMR spectroscopy
- Conserved lipid and small molecule modulation of COQ8 reveals regulation of the ancient UbiB family
- Multi-omic mitoprotease profiling defines a role for Oct1p in coenzyme Q production
- Conserved lipid and small molecule modulation of COQ8 reveals regulation of the ancient kinase-like UbiB family
- Function and solution structure of the Arabidopsis thaliana RALF8 peptide
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