Two-photon excitation STED microscopy.
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Summary
Sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy is reported by merging this technique with stimulated-emission depletion (STED) and an easy-to-implement and promising laser combination based on a short-pulse laser source for two- phytochemical excitation and a continuous-wave (CW) laser sources for resolution enhancement is demonstrated.
- Type
- dissertation
- Published
- 2009-08-17
- Cited by
- 224
- References
- 21
- Access
- Open access
- OpenAlex
- https://openalex.org/W19687936
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:26643577
Keywords
Mathematics education, Foreign language, Pedagogy, Psychology, Transition (genetics)
References
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- Continuous wave excitation two‐photon fluorescence microscopy exemplified with the 647‐nm ArKr laser line
- STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
- Microscopy and its focal switch
- Video-Rate Far-Field Optical Nanoscopy Dissects Synaptic Vesicle Movement
- STED microscopy reveals crystal colour centres with nanometric resolution.
- Principles of two-photon excitation microscopy and its applications to neuroscience.
- Two-photon laser scanning fluorescence microscopy.
- Two-photon near- and far-field fluorescence microscopy with continuous-wave excitation.
- Stimulated-emission-depletion microscopy with a multicolor stimulated-Raman-scattering light source.
- STED microscopy with a supercontinuum laser source.
- 2,2′‐Thiodiethanol: A new water soluble mounting medium for high resolution optical microscopy
- Macromolecular-scale resolution in biological fluorescence microscopy.
- Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.
- Two-photon fluorescence excitation and related techniques in biological microscopy
Cited by
- SUPERRESOLUTION NONLINEAR STRUCTURED ILLUMINATION MICROSCOPY BY STIMULATED EMISSION DEPLETION
- Upgrade of a Scanning Confocal Microscope to a Single-Beam Path STED Microscope
- STED microscopy for nanoscale imaging in living brain slices.
- Nanoscopy inside living brain slices.
- Rapid Two-Photon Holographic Scan Microscopy with Individual Quantum Dots and Its Applications in Biological Samples
- Fluorescence microscopy—A historical and technical perspective
- Photo-induced dark states influorescence spectroscopy – investigations a applications
- 3D super-resolved in vitro multiphoton microscopy by saturation of excitation.
- Super‐Resolution Microscopy: A Comparative Treatment
- Magnetic field effects on molecular emissivity in solutions
- Using photo-instability to quantify fluorophores and achieve super-resolution imaging
- Computational modeling of stimulated emission depletion microscopy in biological cells under one- and two-photon excitation
- Development and application of two-photon excitation stimulated emission depletion microscopy for superresolution fluorescence imaging in thick tissue
- Improvement of the spatial resolution in multiphoton microscopy by saturated excitation of fluorescence
- Axial resolution beyond the diffraction limit of a sheet illumination microscope with stimulated emission depletion
- High-Resolution Intravital Microscopy
- Super-resolution imaging in live cells
- All-optical fluorescence image recovery using modulated Stimulated Emission Depletion
- Strategies to maximize the performance of a STED microscope.
- Subdiffraction-limit two-photon fluorescence microscopy for GFP-tagged cell imaging.
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