Resolution scaling in STED microscopy.
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Summary
A comprehensive study of the inverse square root dependence of spatial resolution on the saturation factor in stimulated emission depletion (STED) microscopy is undertaken and the generalized square-root law is shown to predict the practical resolution with high accuracy.
- Type
- article
- Published
- 2008-03-17
- Cited by
- 434
- References
- 26
- Access
- Open access
- OpenAlex
- https://openalex.org/W18542512
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:35158259
Keywords
Islam, Law, Political science, Family law, History
References
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- STED microscopy with continuous wave beams
- Nanoscale resolution in the focal plane of an optical microscope.
- Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM)
- Anatomy and Dynamics of a Supramolecular Membrane Protein Cluster
- STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis
- Efficient fluorescence inhibition patterns for RESOLFT microscopy.
- Wide-field subdiffraction imaging by accumulated binding of diffusing probes
- Focal spots of size lambda/23 open up far-field fluorescence microscopy at 33 nm axial resolution.
- Mechanism of formation of two-dimensional crystals from latex particles on substrates
- Toward fluorescence nanoscopy
- Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
- Laser-diode-stimulated emission depletion microscopy
- Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution.
- Ultra-high resolution imaging by fluorescence photoactivation localization microscopy.
- Bruchpilot Promotes Active Zone Assembly, Ca2+ Channel Clustering, and Vesicle Release
- Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy.
- Imaging in systems biology.
- Phase filter enhanced STED-4Pi fluorescence microscopy: theory and experiment
- Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins.
Cited by
- Two-photon excitation STED microscopy.
- Super-resolution imaging of nuclear bodies by STED microscopy.
- Analysis of Rho GTPase-induced localization of nanoscale adhesions using fluorescence nanoscopy.
- Sample preparation for STED microscopy.
- Super resolution optical imaging – image analysis, multicolor development and biological applications
- Characterization of molecular orientation in super-resolution fluorescence microscopy
- Quantitative Microscopy to Measure the Nuclear Architecture
- Photoluminescent diamond nanoparticles as labels in cells: study of their optical properties and investigation of their cellular uptake mechanism
- Développements de microscopies optiques pour l’imagerie super-résolue de nanocristaux de diamant fluorescents comme rapporteurs d’anomalies fonctionnelles du neurone
- Structural Information Content of the Optical Field
- STED microscopy for nanoscale imaging in living brain slices.
- Theoretical assessment of two-dimensional nonlinear structured illumination microscopy based on structured excitation and structured stimulated emission depletion
- Single Marker Switching Nanoscopy with 4Pi Detection: Superior Nanometric 3D Resolution
- Confocal laser scanning microscopy of nanoparticles applied to immunosorbent assays
- High-Resolution Microscopy with Photoswitchable Organic Markers
- Fluorescence depletion properties of insulin-gold nanoclusters.
- Bringing Far-Field Subdiffraction Optical Imaging to Electronically Coupled Optoelectronic Molecular Materials Using Their Endogenous Chromophores.
- Advanced electrodynamic mechanisms for the nanoscale control of light by light
- Dynamic organization of lymphocyte plasma membrane: lessons from advanced imaging methods
- Microstructured suspended core fiber for cylindrical vector beams propagation
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