Novel method for high throughput DNA methylation marker evaluation using PNA-probe library hybridization and MALDI-TOF detection
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Summary
A new assay based on peptide nucleic acid (PNA)-library hybridization and subsequent MALDI-TOF analysis is introduced, which is more specific and flexible than established methods, such as microarrays and MS-SNuPE.
- Type
- book
- Published
- 2006-05-02
- Cited by
- 48
- References
- 49
- Access
- Open access
- OpenAlex
- https://openalex.org/W16670426
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:7800428
Keywords
Forestry, Geography
References
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- The transcription factor Eyes absent is a protein tyrosine phosphatase
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- Rapid identification of promoter hypermethylation in hepatocellular carcinoma by pyrosequencing of etiologically homogeneous sample pools.
- Anion-exchanged nanosolid support of magnetic nanoparticle in combination with PNA probes for DNA sequence analysis
- Identification of body fluid-specific DNA methylation markers for use in forensic science.
- Spatial, temporal and interindividual epigenetic variation of functionally important DNA methylation patterns
- Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development
- Methods for identification of epigenetic elements in mammalian long multigenic genome sequences
- Epigenetic silencing of KAZALD1 confers a better prognosis and is associated with malignant transformation/progression in glioma.
- Maps of cis-Regulatory Nodes in Megabase Long Genome Segments are an Inevitable Intermediate Step Toward Whole Genome Functional Mapping.
- Decision criteria for rational selection of homogeneous genotyping platforms for pharmacogenomics testing in clinical diagnostics
- Genome-wide DNA methylation profiling identifies ALDH1A3 promoter methylation as a prognostic predictor in G-CIMP- primary glioblastoma.
- Peptide nucleic acid probes with charged photocleavable mass markers
- Toward a comprehensive and systematic methylome signature in colorectal cancers
- Matrix-Assisted Laser Desorption/Ionisation, Time-of-Flight Mass Spectrometry in Genomics Research
- Capillary electrophoretic analysis of methylation status in CpG-rich regions by single-base extension of primers modified with N6-methoxy-2,6-diaminopurine.
- The Eyes Absent Proteins in Development and Disease
- Electrospray ionisation-cleavable tandem nucleic acid mass tag–peptide nucleic acid conjugates: synthesis and applications to quantitative genomic analysis using electrospray ionisation-MS/MS
- Mass spectrometry of nucleic acids in molecular medicine
- Direct DNA Methylation Profiling Using Methyl Binding Domain Proteins
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