Lipid‐based strategies used to identify extracellular vesicles in flow cytometry can be confounded by lipoproteins: Evaluations of annexin V, lactadherin, and detergent lysis
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Summary
Whether PS labelling and detergent lysis approaches are confounded by lipoproteins, another family of lipid‐based nanoparticles found in blood, is investigated and the use of transmembrane proteins such as tetraspannins to distinguish EVs from lipoprotein‐bound PS is supported.
- Type
- article
- Published
- 2022-03-31
- Cited by
- 40
- References
- 55
- Access
- Open access
- OpenAlex
- https://openalex.org/W4221021346
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:247854218
Keywords
Phosphatidylserine, Lysis, Flow cytometry, Lipoprotein, Polyclonal antibodies
References
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Cited by
- Extracellular vesicles and lipoproteins – Smart messengers of blood cells in the circulation
- Compositional profiling of EV-lipoprotein mixtures by AFM nanomechanical imaging
- Exosomal lipids from membrane organization to biomarkers: Focus on an endolysosomal-specific lipid.
- Detectable Lipidomes and Metabolomes by Different Plasma Exosome Isolation Methods in Healthy Controls and Patients with Advanced Prostate and Lung Cancer
- Optical microscopic and spectroscopic detection of exosomes
- A new isolation method for bacterial extracellular vesicles providing greater purity and improved proteomic detection of vesicle proteins
- Platelet-Derived Microparticles and Autoimmune Diseases
- Phenotypic and proteomic analysis of plasma extracellular vesicles highlights them as potential biomarkers of primary Sjögren syndrome
- Label‐free discrimination of extracellular vesicles from large lipoproteins
- Quantitative assessment of lipophilic membrane dye‐based labelling of extracellular vesicles by nano‐flow cytometry
- Isolation of Hepatic and Adipose-Tissue-Derived Extracellular Vesicles Using Density Gradient Separation and Size Exclusion Chromatography
- Identification of a Non-Invasive Urinary Exosomal Biomarker for Diabetic Nephropathy Using Data-Independent Acquisition Proteomics
- Particle profiling of EV‐lipoprotein mixtures by AFM nanomechanical imaging
- Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection.
- Physical association of low density lipoprotein particles and extracellular vesicles unveiled by single particle analysis
- Circulating extracellular vesicles in healthy and pathological pregnancies: A scoping review of methodology, rigour and results
- Expression of Tissue Factor and Platelet/Leukocyte Markers on Extracellular Vesicles Reflect Platelet–Leukocyte Interaction in Severe COVID-19
- MIBlood‐EV: Minimal information to enhance the quality and reproducibility of blood extracellular vesicle research
- Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
- Exploring the role of epicardial adipose-tissue-derived extracellular vesicles in cardiovascular diseases
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