Effective Refractive Index and Lipid Content of Extracellular Vesicles Revealed Using Optical Waveguide Scattering and Fluorescence Microscopy.
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Summary
This work uses surface-sensitive waveguide scattering microscopy with single EV resolution to characterize two subsets of similarly sized EVs that were preseparated based on their difference in buoyant density, and suggests that separation in density gradients is more likely because of the differences in membrane permeability than to differences in biomolecular content of the EVs.
- Type
- article
- Published
- 2018-06-20
- Cited by
- 31
- References
- 64
- Access
- Open access
- OpenAlex
- https://openalex.org/W29923735
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:49314294
Keywords
Computer science
References
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- Size and Refractive Index determination of Sub-Wavelength Particles and Air Bubbles by Holographic Nanoparticle Tracking Analysis.
- Independent Size and Fluorescence Emission Determination of Individual Biological Nanoparticles Reveals that Lipophilic Dye Incorporation Does Not Scale with Particle Size.
- Precise nanosizing with high dynamic range holography
- Small extracellular vesicles (sEVs): discovery, functions, applications, detection methods and various engineered forms
- Cell refractive index: Models, insights, applications and future perspectives.
- Fast and Accurate Nanoparticle Characterization Using Deep-Learning-Enhanced Off-Axis Holography
- Refractive index of biological tissues: Review, measurement techniques, and applications.
- Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins
- Lipid‐based strategies used to identify extracellular vesicles in flow cytometry can be confounded by lipoproteins: Evaluations of annexin V, lactadherin, and detergent lysis
- Protein A/G-Based Surface Plasmon Resonance Biosensor for Regenerable Antibody-Mediated Capture and Analysis of Nanoparticles
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- The Influence of Nanobubble Size and Stability on Ultrasound Enhanced Drug Delivery
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- Tracking the Dissolution Surface Kinetics of a Single Fluorescent Cyclodextrin Metal-Organic Framework by Confocal Laser Scanning Microscopy.
- High-angle deflection of metagrating-integrated laser emission for high-contrast microscopy
- Inflammatory pain resolution by mouse serum-derived small extracellular vesicles
- Chiroptical detection and mutation analysis of cancer-associated extracellular vesicles using microfluidics with oriented chiral nanoparticles
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