CRISPR-Cas9 Delivery with the Ribonucleoprotein Complexes Increased EGFP Editing Efficiency
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Summary
It was showed that the efficiency of single nucleotide correction in the EGFP gene at the plasmid locus significantly increased to 35% when using RNP instead of plasmids, which may form the basis for the development of methods of correction of disease-causing genetic mutations.
- Type
- article
- Published
- 2021-10-01
- Cited by
- 0
- References
- 19
- OpenAlex
- https://openalex.org/W4200108880
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:245228616
Keywords
CRISPR, Cas9, Ribonucleoprotein, Genome editing, Plasmid
References
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- High-fidelity CRISPR-Cas9 variants with undetectable genome-wide off-targets
- Improved delivery of Cas9 protein/gRNA complexes using lipofectamine CRISPRMAX
- Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
- Non-viral delivery of genome-editing nucleases for gene therapy
- Efficient Gene Editing through Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein
- A highly specific SpCas9 variant is identified by in vivo screening in yeast
- Enhanced Cytosolic Delivery and Release of CRISPR/Cas9 by Black Phosphorus Nanosheets for Genome Editing.
- A boronic acid–rich dendrimer with robust and unprecedented efficiency for cytosolic protein delivery and CRISPR-Cas9 gene editing
- Search-and-replace genome editing without double-strand breaks or donor DNA
- Carboxylated branched poly(β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing
- Engineering of Monosized Lipid-Coated Mesoporous Silica Nanoparticles for CRISPR Delivery.
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