Endocytic Profiling of Cancer Cell Models Reveals Critical Factors Influencing LNP-Mediated mRNA Delivery and Protein Expression.
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Summary
It is established that efficient LNP-mRNA transfection relies on an early and narrow endosomal escape window prior to lysosomal sequestration and/or exocytosis, and should form an important pre-clinical evaluation step for nucleic acid delivery systems.
- Type
- article
- Published
- 2019-11-01
- Cited by
- 106
- References
- 47
- Access
- Open access
- OpenAlex
- https://openalex.org/W2964528532
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:201099398
Keywords
Endocytic cycle, Endosome, Transfection, Cell biology, Nucleic acid
References
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- Advances in Lipid Nanoparticles for siRNA Delivery
- The pH of the digestive vacuole of Plasmodium falciparum is not associated with chloroquine resistance
- Non-viral vectors for gene-based therapy
- Rab7 Associates with Early Endosomes to Mediate Sorting and Transport of Semliki Forest Virus to Late Endosomes
- Pathways and mechanisms of endocytic recycling
- Expression of therapeutic proteins after delivery of chemically modified mRNA in mice
- Endocytic delivery to lysosomes mediated by concurrent fusion and kissing events in living cells.
- State-of-the-art gene-based therapies: the road ahead
Cited by
- Ionizable lipid nanoparticles encapsulating barcoded mRNA for accelerated in vivo delivery screening
- An efficient polymer pendant approach towards high stable organic fluorophore for sensing ultra-trace Hg2+ with improved biological compatibility and cell permeability.
- Quantitating Endosomal Escape of a Library of Polymers for mRNA Delivery
- Optimizing synthetic nucleic acid and protein nanocarriers: The chemical evolution approach.
- Arrayed CRISPR Screening Identifies Novel Targets That Enhance the Productive Delivery of mRNA by MC3-Based Lipid Nanoparticles
- Formulation and Delivery Technologies for mRNA Vaccines
- Lipid nanoparticle technology for therapeutic gene regulation in the liver
- Barriers and Strategies of Cationic Liposomes for Cancer Gene Therapy
- An investigation of rhinovirus infection on cellular uptake of poly (glycerol-adipate) nanoparticles
- Effect of complexing lipids on cellular uptake and expression of messenger RNA in human skin explants.
- DOPC versus DOPE as a helper lipid for gene-therapies: molecular dynamics simulations with DLin-MC3-DMA.
- The Role of cis- and trans-Acting RNA Regulatory Elements in Leukemia
- Cytosolic delivery of nucleic acids: The case of ionizable lipid nanoparticles
- A high-throughput Galectin-9 imaging assay for quantifying nanoparticle uptake, endosomal escape and functional RNA delivery
- Highway to Success—Developing Advanced Polymer Therapeutics
- The dawn of mRNA vaccines: The COVID-19 case
- [New Hopes in Vaccine Technology: mRNA Vaccines].
- Impact of Ribosome Activity on SARS-CoV-2 LNP – Based mRNA Vaccines
- mRNA Delivery of a Bispecific Single Domain Antibody to Polarize Tumor-associated Macrophages and Synergize Immunotherapy Against Liver Malignancies
- Escaping the endosome: Assessing cellular trafficking mechanisms of non-viral vehicles.
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