Efficient Production of On-Target Reads for Small RNA Sequencing of Single Cells Using Modified Adapters
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Summary
Two methods of single-cell-lysis that enable sc-sRNA-seq are presented and it is anticipated this approach will create a new opportunity to explore cellular heterogeneity through small RNA expression.
- Type
- article
- Published
- 2018-09-27
- Cited by
- 13
- References
- 26
- Access
- Open access
- OpenAlex
- https://openalex.org/W2893961808
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:52842763
Keywords
RNA, Chemistry, Transfer RNA, Small RNA, Adapter (computing)
References
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- Deep sequencing of small RNA transcriptome reveals novel non-coding RNAs in hepatocellular carcinoma.
- Isotachophoresis for fractionation and recovery of cytoplasmic RNA and nucleus from single cells
- Deciphering targeting rules of splicing modulator compounds: case of TG003
- On-chip separation and analysis of RNA and DNA from single cells.
- Protocol for Microfluidic System to Automate the Preparation and Fractionation of the Nucleic Acids in the Cytoplasm Versus Nuclei of Single Cells
- SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation
- Next-generation sequencing-based small RNA profiling of cerebrospinal fluid exosomes.
- Single-cell sequencing of the small-RNA transcriptome
- Small RNA Library Preparation Method for Next-Generation Sequencing Using Chemical Modifications to Prevent Adapter Dimer Formation
- Single-cell barcoding and sequencing using droplet microfluidics
- Single-Cell Analysis of Circulating Tumor Cells as a Window into Tumor Heterogeneity
Cited by
- Simultaneous RNA purification and size selection using on-chip isotachophoresis with an ionic spacer
- Isolation of enriched small RNA from cell‐lysate using on‐chip isotachophoresis
- Distinct kinetics in electrophoretic extraction of cytoplasmic RNA from single cells.
- Isotachophoresis-Enhanced Immunoassays: Challenges and opportunities
- Length bias-free extraction of cytoplasmic RNA from single cells by electrical lysis and electrophoresis
- Targeted permeabilization of the cell wall and extraction of charged molecules from single cells in intact plant clusters using a focused electric field.
- NanoSINC-seq dissects the isoform diversity in subcellular compartments of single cells
- Single cell quantification of ribosome occupancy in early mouse development
- Liver in infections: a single-cell and spatial transcriptomics perspective
- Offline preparative separation methods based on electromigration: An overview and current trends
- Untacking small RNA profiling and RNA fragment footprinting: Approaches and challenges in library construction
- Inferring single-cell and spatial microRNA activity from transcriptomics data
- Inferring single-cell and spatial microRNA activity from transcriptomics data
- Future scope of small RNA technology in crop science
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