A comparison of methods used to unveil the genetic and metabolic pool in the built environment
Explore this paper's citation graph
Summary
In an environment that has primarily dead and metabolically inactive cells, it is suggested that the rRNA fraction of BE samples is capable of providing a more ecologically relevant insight into the factors that drive indoor microbial community dynamics.
- Type
- article
- Published
- 2018-04-16
- Cited by
- 24
- References
- 81
- Access
- Open access
- OpenAlex
- https://openalex.org/W2805170795
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:4945910
Keywords
Biology, Ribosomal RNA, DNA extraction, 18S ribosomal RNA, Microbial ecology
References
- Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics
- Indoor air bacterial communities in Hong Kong households assemble independently of occupant skin microbiomes.
- Chamber Bioaerosol Study: Outdoor Air and Human Occupants as Sources of Indoor Airborne Microbes
- Thermal injury and recovery of Bacillus subtilis.
- ggplot2: Elegant Graphics for Data Analysis
- Sparse and Compositionally Robust Inference of Microbial Ecological Networks
- Humans differ in their personal microbial cloud
- New perspectives on viable microbial communities in low-biomass cleanroom environments
- Evaluating rRNA as an indicator of microbial activity in environmental communities: limitations and uses
- Evaluation of DNAstable for DNA storage at ambient temperature.
- The Diversity and Distribution of Fungi on Residential Surfaces
- Protocols for dry DNA storage and shipment at room temperature
- Quantitative response of nitrifying and denitrifying communities to environmental variables in a full-scale membrane bioreactor.
- Temporal Dynamics of Active Prokaryotic Nitrifiers and Archaeal Communities from River to Sea
- Comparison of surface sampling methods and cleanability assessment of stainless steel surfaces subjected or not to shot peening
- When stable RNA becomes unstable: the degradation of ribosomes in bacteria and beyond
- Activity of abundant and rare bacteria in a coastal ocean
- Microbial seed banks: the ecological and evolutionary implications of dormancy
- Indoor-Air Microbiome in an Urban Subway Network: Diversity and Dynamics
- Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction
Cited by
- Rare Taxa Exhibit Disproportionate Cell-Level Metabolic Activity in Enriched Anaerobic Digestion Microbial Communities
- Building upon current knowledge and techniques of indoor microbiology to construct the next era of theory into microorganisms, health, and the built environment
- RNA-based qPCR as a tool to quantify and to characterize dual-species biofilms
- Evaluation of the microbial community structure of potable water samples from occupied and unoccupied buildings using16S rRNA amplicon sequencing
- The environmental risk assessment of cell-processing facilities for cell therapy in a Japanese academic institution
- Prevalence of methanogens in the uncultured Sikkim hot spring solfataric mud archaeal microbiome
- Toward Accurate and Robust Environmental Surveillance Using Metagenomics
- Towards reproducible metabarcoding data: Lessons from an international cross‐laboratory experiment
- City-Scale Meta-Analysis of Indoor Airborne Microbiota Reveals that Taxonomic and Functional Compositions Vary with Building Types.
- Where environmental microbiome meets its host: Subway and passenger microbiome relationships
- Metagenomics vs. total RNA sequencing: most accurate data-processing tools, microbial identification accuracy, and implications for freshwater assessments
- Metagenomics versus total RNA sequencing: most accurate data-processing tools, microbial identification accuracy and perspectives for ecological assessments
- Development of sequencing-based methodologies to distinguish viable from non-viable cells in a bovine milk matrix: A pilot study
- An improved workflow for accurate and robust healthcare environmental surveillance using metagenomics
- Functional gene transcription variation in bacterial metatranscriptomes in large freshwater Lake Ecosystems: Implications for ecosystem and human health.
- RNA-based amplicon sequencing is ineffective in measuring metabolic activity in environmental microbial communities
- Shallow and deep groundwater moderate methane dynamics in a high Arctic glacial catchment
- The indoors microbiome and human health
- Host species and temperature drive beech and Scots pine phyllosphere microbiota across European forests
- From air to insight: the evolution of airborne DNA sequencing technologies
Related papers
- بررسی پایداری بیان ژنهای مرجع ovEF1alpha و 18S rRNA تحت تأثیر تیمارهای جیبرلین و تنشهای دما و نور در جنس آویشن
- Letter to Microbial Ecology
- Rhizobium albus sp. nov., Isolated from Lake Water in Xiamen, Fujian Province of China
- The FibRumBa Database: A Resource for Biologists with Interests in Gastrointestinal Microbial Ecology, Plant Biomass Degradation, and Anaerobic Microbiology
- Nematode Diversity of Qingdao Coast Inferred from the 18S Ribosomal RNA Gene Sequence Analysis
- First successful amplification of 18S ribosomal DNA ofCordyceps spp. by the PCR method
- Phylogenetic Relationships Among Selected Heteroderoidea Based on 18S and ITS Ribosomal DNA.
- Organization of the nuclear ribosomal DNA of Chlamydomonas reinhardii
- Variable arrangement of 5S ribosomal genes within the ribosomal DNA repeats of arthropods.