Routine Determination of Ice Thickness for Cryo-EM Grids
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Summary
Two methods, using either an energy filter or scattering outside the objective aperture, to measure ice thickness for potentially every image collected, and should prove helpful for optimizing sample preparation, data collection, and data processing.
- Type
- preprint
- Published
- 2018-04-16
- Cited by
- 144
- References
- 38
- Access
- Open access
- OpenAlex
- https://openalex.org/W2797085332
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:51598926
Keywords
Workflow, Data collection, Computer science, Automation, Sample (material)
References
- Asynchronous data acquisition and on-the-fly analysis of dose fractionated cryoEM images by UCSFImage
- Automated batch fiducial-less tilt-series alignment in Appion using Protomo
- Electron microscopy: Ultrastable gold substrates for electron cryomicroscopy.
- Cryo-electron microscopy of viruses
- Elastic and inelastic scattering cross-sections of amorphous layers of carbon and vitrified ice
- Determination of the inelastic mean free path of electrons in vitrified ice layers for on‐line thickness measurements by zero‐loss imaging
- Image formation modeling in cryo-electron microscopy.
- Determination of the inelastic mean free path in ice by examination of tilted vesicles and automated most probable loss imaging.
- Spotiton: A prototype for an integrated inkjet dispense and vitrification system for cryo-TEM
- Automated molecular microscopy: the new Leginon system.
- Fully automated, sequential tilt-series acquisition with Leginon
- Automated electron microscope tomography using robust prediction of specimen movements.
- 2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy
- SPARX, a new environment for Cryo-EM image processing.
- CTFFIND4: Fast and accurate defocus estimation from electron micrographs
- Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300kV.
- Appion: an integrated, database-driven pipeline to facilitate EM image processing
- Cryo-electron energy loss spectroscopy: observations on vitrified hydrated specimens and radiation damage.
- Simultaneous determination of sample thickness, tilt, and electron mean free path using tomographic tilt images based on Beer-Lambert law
- Single-particle cryo-EM data acquisition by using direct electron detection camera.
Cited by
- Benchmarking cryo-EM Single Particle Analysis Workflow
- The energy dependence of contrast and damage in electron cryomicroscopy of biological molecules
- A new cryo-EM system for electron 3D crystallography by eEFD
- Study on sustainable development of power transmission system under ice disaster based on a new security early warning model
- Subtomogram averaging from cryo-electron tomograms.
- Reducing effects of particle adsorption to the air-water interface in cryoEM
- Software tools for automated Transmission Electron Microscopy
- cryo_fit: Democratization of Flexible Fitting for Cryo-EM
- Best Practices at the National Center for CryoEM Access and Training
- Live analysis and reconstruction of single-particle cryo-electron microscopy data with CryoFLARE
- Structure and Drug Resistance of the Plasmodium falciparum Transporter PfCRT
- Robust ultraclean atomically thin membranes for atomic-resolution electron microscopy
- Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs.
- What Could Go Wrong? A Practical Guide To Single-Particle Cryo-EM: From Biochemistry To Atomic Models
- Hybrid Vesicle Stability under Sterilisation and Preservation Processes Used in the Manufacture of Medicinal Formulations
- Structural and Functional Characterization of the Bestrophin-2 Anion Channel
- Label-free single-instance protein detection in vitrified cells
- Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different Timescales
- The National Cryo-EM Facility at Frederick National Laboratory: Operational Procedures, Methods and Outputs
- Mechanism of ligand activation of a eukaryotic cyclic nucleotide-gated channel
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