Whole-Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution.
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Summary
Two detailed protocols based on commercial kits for the preparation of sequencing libraries for the comprehensive whole-genome analysis of DNA methylation and/or hydroxymethylation are provided and yield robust and reproducible results.
- Type
- article
- Published
- 2018-01-01
- Cited by
- 35
- References
- 44
- Access
- Open access
- OpenAlex
- https://openalex.org/W2789355158
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:3784675
Keywords
Bisulfite sequencing, Methylated DNA immunoprecipitation, Bisulfite, DNA methylation, Illumina Methylation Assay
References
- Epigenetic changes in inflammatory and autoimmune diseases.
- Epigenetic Mechanisms in Schizophrenia
- DNA methylation as a diagnostic and therapeutic target in the battle against Type 2 diabetes.
- Epigenetics in autoimmune diseases: Pathogenesis and prospects for therapy.
- The epigenetics of aging and neurodegeneration
- An update on the epigenetics of psychotic diseases and autism.
- The Honey Bee Epigenomes: Differential Methylation of Brain DNA in Queens and Workers
- Early allopolyploid evolution in the post-Neolithic Brassica napus oilseed genome
- Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells
- MethylC-seq library preparation for base-resolution whole-genome bisulfite sequencing
- Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine
- Active DNA demethylation: many roads lead to Rome
- Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine
- DNA methylation based biomarkers: practical considerations and applications.
- Hotspots of aberrant epigenomic reprogramming in human induced pluripotent stem cells
- Contribution of Intragenic DNA Methylation in Mouse Gametic DNA Methylomes to Establish Oocyte-Specific Heritable Marks
- Quantitative Sequencing of 5-Methylcytosine and 5-Hydroxymethylcytosine at Single-Base Resolution
- Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation
- Methodological aspects of whole-genome bisulfite sequencing analysis
- A decade of exploring the cancer epigenome — biological and translational implications
Cited by
- Highly sensitive detection of DNA hypermethylation in melanoma cancer cells
- The Impact of Next-Generation Sequencing on Cancer Genomics: From Discovery to Clinic.
- Recent advances in neuroepigenetic editing
- The State-of-the Art of Environmental Toxicogenomics: Challenges and Perspectives of “Omics” Approaches Directed to Toxicant Mixtures
- DNA Methylation Tools and Strategies: Methods in a Review
- Comparison of commercially available whole-genome sequencing kits for variant detection in circulating cell-free DNA
- Analysis of DNA methylation differences in gonads of the large yellow croaker.
- Aberrantly Methylated cfDNA in Body Fluids as a Promising Diagnostic Tool for Early Detection of Breast Cancer.
- Modern Epigenetics Methods in Biological Research
- mRNA expression and DNA methylation analysis of the inhibitory mechanism of H2O2 on the proliferation of A549 cells
- DNA methylation methods: global DNA methylation and methylomic analyses.
- Role of Transposable Elements in Gene Regulation in the Human Genome
- Cancer Biomarkers Discovery of Methylation Modification With Direct High-Throughput Nanopore Sequencing
- Focal disruption of DNA methylation dynamics at enhancers in IDH mutant AML cells
- Multi-omics comparison of malignant and normal uveal melanocytes reveals novel molecular features of uveal melanoma
- DNA methylation as a mediator of genetic and environmental influences on Parkinson’s disease susceptibility: Impacts of alpha-Synuclein, physical activity, and pesticide exposure on the epigenome
- Environmental Adaptation of Genetically Uniform Organisms with the Help of Epigenetic Mechanisms—An Insightful Perspective on Ecoepigenetics
- Uropathogenic Escherichia coli infection-induced epithelial trained immunity impacts urinary tract disease outcome
- DNA methylation changes underlie the long-term association between periodontitis and atherosclerotic cardiovascular disease
- Novel insights into systemic sclerosis using a sensitive computational method to analyze whole-genome bisulfite sequencing data
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