Tools to investigate the ubiquitin proteasome system.
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Summary
Many proteins involved in either the ubiquitination, deubiquitination or degradation of proteins are implicated in human diseases and are currently under investigation as potential drug targets.
- Type
- review
- Published
- 2017-12-01
- Cited by
- 76
- References
- 44
- Access
- Open access
- OpenAlex
- https://openalex.org/W2770653741
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:23022648
Keywords
Deubiquitinating enzyme, Ubiquitin, Proteasome, Ubiquitin-conjugating enzyme, Ubiquitin ligase
References
- Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis.
- Monitoring activity and inhibition of 26S proteasomes with fluorogenic peptide substrates.
- Selective Target Protein Degradation via Phthalimide Conjugation
- The synthesis and proteasomal degradation of a model substrate Ub5DHFR.
- Short-lived green fluorescent proteins for quantifying ubiquitin/proteasome-dependent proteolysis in living cells
- A Set of Activity-Based Probes to Visualize Human (Immuno)proteasome Activities.
- A fluorescent broad-spectrum proteasome inhibitor for labeling proteasomes in vitro and in vivo.
- Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation
- A Native Chemical Ligation Handle that Enables the Synthesis of Advanced Activity-Based Probes: Diubiquitin as a Case Study
- Quantitative analysis of in vitro ubiquitinated cyclin B1 reveals complex chain topology
- Sensitive detection of proteasomal activation using the Deg-On mammalian synthetic gene circuit
- Disassembly of Lys11 and Mixed Linkage Polyubiquitin Conjugates Provides Insights into Function of Proteasomal Deubiquitinases Rpn11 and Ubp6*
- Protein knockdown using methyl bestatin-ligand hybrid molecules: design and synthesis of inducers of ubiquitination-mediated degradation of cellular retinoic acid-binding proteins.
- Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format.
- Designed Semisynthetic Protein Inhibitors of Ub/Ubl E1 Activating Enzymes
- Activity probe for in vivo profiling of the specificity of proteasome inhibitor bortezomib
- Regulation of proteasome activity in health and disease
- Generation of Destabilized Green Fluorescent Protein as a Transcription Reporter*
- Deubiquitinating Enzyme Specificity for Ubiquitin Chain Topology Profiled by Di-Ubiquitin Activity Probes
- Peptide and small molecule inhibitors of HECT-type ubiquitin ligases
Cited by
- Characterizing disease-associated changes in post-translational modifications by mass spectrometry
- Calcium/calmodulin‐dependent kinase II and memory destabilization: a new role in memory maintenance
- Regulation of Protein Degradation and Homeostasis by the Cytokine- Inducible Deubiquitinating Enzymes
- Semi-synthesis of Ubiquitin-propargylamide for identifying deubiquitinase targeting inhibitors
- Cellular mechanisms promoting cachexia and how they are opposed by sirtuins 1.
- Recombinant HCV NS3 and NS5B enzymes exhibit multiple posttranslational modifications for potential regulation
- The E3 ubiquitin ligase HECW1 targets thyroid transcription factor 1 (TTF1/NKX2.1) for its degradation in the ubiquitin-proteasome system
- Neurodegeneration meets immunology - A chemical biology perspective.
- c-Cbl: An Important Regulator and a Target in Angiogenesis and Tumorigenesis
- TRIM50 acts as a novel Src suppressor and inhibits ovarian cancer progression.
- Lipid peroxidation in neurodegeneration.
- Proteasoma, subtipos y sus implicaciones en la tolerancia central
- Control of the inflammasome by the ubiquitin system
- Ubiquitylomes Analysis of the Whole blood in Postmenopausal Osteoporosis Patients and healthy Postmenopausal Women
- Recent Developments in Cell Permeable Deubiquitinating Enzyme Activity-Based Probes
- RING Finger Protein 38 Mediates LIM Domain Binding 1 Degradation and Regulates Cell Growth in Colorectal Cancer
- The aberrant expression of ADAR1 promotes resistance to BET inhibitors in pancreatic cancer by stabilizing c-Myc.
- UbcH10 a Major Actor in Cancerogenesis and a Potential Tool for Diagnosis and Therapy
- Cellular Functions of OCT-3/4 Regulated by Ubiquitination in Proliferating Cells
- Strategies to investigate protein turnover with fluorescent protein reporters in eukaryotic organisms
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