Slowed Diffusion and Excluded Volume Both Contribute to the Effects of Macromolecular Crowding on Alcohol Dehydrogenase Steady-State Kinetics.
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Summary
Evidence is presented that other factors, in addition to excluded volume, must be considered in the interpretation of crowding studies with synthetic polymers, as additional effects from the underlying monomer need to be considered.
- Type
- article
- Published
- 2015-09-16
- Cited by
- 24
- References
- 62
- OpenAlex
- https://openalex.org/W2230106831
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:19378666
Keywords
Macromolecular crowding, Kinetics, Alcohol dehydrogenase, Diffusion, Excluded volume
References
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Cited by
- Effects of Macromolecular Crowding on Alcohol Dehydrogenase Activity Are Substrate-Dependent.
- Large cosolutes, small cosolutes, and dihydrofolate reductase activity
- Consequences of Heterogeneous Crowding on an Enzymatic Reaction: A Residence Time Monte Carlo Approach
- Lipid composition and macromolecular crowding effects on CYP2J2‐mediated drug metabolism in nanodiscs
- When both Km and Vmax are altered, Is the enzyme inhibited or activated?
- Effects of Macromolecular Crowding on Biochemical Systems
- Catalytic studies of glutathione transferase from Clarias gariepinus (Burchell) in dilute and crowded solutions.
- Overlap Concentration and the effect of Macromolecular Crowding on Citrate Synthase Activity.
- CYP2J2 Molecular Recognition: A New Axis for Therapeutic Design
- Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation
- Macromolecular crowding effects on the kinetics of opposing reactions catalyzed by alcohol dehydrogenase
- Stochastic pH Oscillations in a Model of the Urea-Urease Reaction Confined to Lipid Vesicles.
- Cosolutes Modify Alkaline Phosphatase Catalysis through Osmotic Stress and Crowding Mechanisms
- Protein Dynamics as a Sensor for Macromolecular Crowding: Insights into Mixed Crowding
- Biomolecular Condensates Regulate Enzymatic Activity under a Crowded Milieu: Synchronization of Liquid-Liquid Phase Separation and Enzymatic Transformation.
- Effect of crowding, compartmentalization and nanodomains on protein modification and redox signaling - current state and future challenges.
- Kinetic Study of the Esterase-like Activity of Albumin following Condensation by Macromolecular Crowding.
- Biomolecular Condensation of Trypsin Prevents Autolysis and Promotes Ca2+-Mediated Activation of Esterase Activity
- Affinity‐Controlled Partitioning of Biomolecules at Aqueous Interfaces and Their Bioanalytic Applications
- Role of Associated Water Dynamics on Protein Stability and Activity in Crowded Milieu.
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