Genetic applications of an inverse polymerase chain reaction.
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Summary
The feasibility of IPCR is shown by amplifying the sequences that flank an IS1 element in the genome of a natural isolate of Escherichia coli.
- Type
- article
- Published
- 1988-11-01
- Cited by
- 1,903
- References
- 9
- Access
- Open access
- OpenAlex
- https://openalex.org/W2159686532
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:11028648
Keywords
Inverse polymerase chain reaction, Biology, Transposable element, Polymerase chain reaction, Genetics
References
- Standard reference strains of Escherichia coli from natural populations
- Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.
- Distribution and abundance of insertion sequences among natural isolates of Escherichia coli.
- Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.
- Direct cloning and sequence analysis of enzymatically amplified genomic sequences.
- Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
- Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
- DNA typing from single hairs
- Nucleotide sequence of an insertion element, IS1.
- Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
Cited by
- A biochemical perspective of the polymerase chain reaction.
- メタゲノムからの新規遺伝子探索 : PCRを用いた様々なアプローチ( 遺伝資源としての土壌微生物研究法の新展開)
- Human somatic gene therapy: progress and problems
- Genome-Wide Transposon Mutagenesis in Saccharomyces cerevisiae and Candida albicans
- Complete sequence of omp1, the structural gene encoding the 40-kDa outer membrane protein of Fusobacterium nucleatum strain Fev1.
- Experimental analysis of global gene regulation in Escherichia coli.
- An alternative linker-mediated polymerase chain reaction method using a dideoxynucleotide to reduce amplification background.
- Salmonella typhimurium Virulence Genes Are Induced upon Bacterial Invasion into Phagocytic and Nonphagocytic Cells
- Monosomy for the most telomeric, gene-rich region of the short arm of human chromosome 16 causes minimal phenotypic effects
- 食品検体の腸管出血性大腸菌O157・O26汚染一次スクリーニング用Multiplex PCR法の開発
- SegG endonuclease promotes marker exclusion and mediates co-conversion from a distant cleavage site.
- Transcription of σ54‐dependent but not σ28‐dependent flagellar genes in Campylobacter jejuni is associated with formation of the flagellar secretory apparatus
- HybF, a Zinc-Containing Protein Involved in NiFe Hydrogenase Maturation
- Amplification of genomic sequences flanking T-DNA insertions by thermal asymmetric interlaced polymerase chain reaction.
- Development of real-time PCR using Minor Groove Binding probe to monitor the biological control agent Candida oleophila (strain O).
- Development of a highly efficient gene targeting system for Fusarium graminearum using the disruption of a polyketide synthase gene as a visible marker.
- Identification of a Nicotiana plumbaginifolia Plasma Membrane H+-ATPase Gene Expressed in the Pollen Tube
- Molecular breakpoint analysis of chromosome translocations in cancer cell lines by Long Distance Inverse-PCR.
- Stereoselective synthesis of (R)-3-quinuclidinol through asymmetric reduction of 3-quinuclidinone with 3-quinuclidinone reductase of Rhodotorula rubra
- Phylogenetic analysis of heavy-metal ATPases in fungi and characterization of the copper-transporting ATPase of Cochliobolus heterostrophus.
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- OUP accepted manuscript