Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking.
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Summary
This work has developed a method that allows the amplification of double-stranded DNA even when the sequence information is available at one end only, and allows unidirectional genome walking from known into unknown regions of the chromosome.
- Type
- article
- Published
- 1993-01-01
- Cited by
- 16
- References
- 7
- OpenAlex
- https://openalex.org/W1564541111
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:33189890
Keywords
Hot start PCR, In silico PCR, Multiple displacement amplification, Polymerase chain reaction, Primer (cosmetics)
References
- Molecular analysis of X‐autosome translocations in females with Duchenne muscular dystrophy.
- Experiments in molecular genetics
- Amplification of bacterial genomic DNA by the polymerase chain reaction and direct sequencing after asymmetric amplification: application to the study of periplasmic permeases
- Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.
- Use of a simplified single-site PCR to facilitate cloning of genomic DNA sequences flanking a transgene integration site.
- Genome walking by single-specific-primer polymerase chain reaction: SSP-PCR.
- Tandem chromosomal duplications: role of REP sequences in the recombination event at the join‐point.
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