Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy.
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Summary
The hand determination and refinement optimization procedure is applied to image pairs of the dihydrolipoyl acetyltransferase (E2) catalytic core of the pyruvate dehydrogenase complex from Bacillus stearothermophilus taken by low-dose electron cryomicroscopy.
- Type
- article
- Published
- 2003-10-31
- Cited by
- 2,412
- References
- 45
- OpenAlex
- https://openalex.org/W2152562710
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:33883985
Keywords
Single particle analysis, Cryo-electron microscopy, Tilt (camera), Particle (ecology), Orientation (vector space)
References
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- Containment system for the preparation of vitrified-hydrated virus specimens.
- Molecular architecture and mechanism of an icosahedral pyruvate dehydrogenase complex: a multifunctional catalytic machine
- A method for establishing the handedness of biological macromolecules.
- Mean phase error and the map-correlation coefficient.
- Holey Films for Electron Microscopy
- The CCP4 suite: programs for protein crystallography.
- A maximum-likelihood approach to single-particle image refinement.
- Improved methods for building protein models in electron density maps and the location of errors in these models.
- Structure of keyhole limpet hemocyanin type 1 (KLH1) at 15 A resolution by electron cryomicroscopy and angular reconstitution.
- Structure of viruses of the papilloma-polyoma type. IV. Analysis of tilting experiments in the electron microscope.
- Experimental High-Resolution Electron Microscopy
- Evaluation of charging on macromolecules in electron cryomicroscopy.
- Similarity measures between images
- Determination of Absolute from Relative X-Ray Intensity Data
Cited by
- Protein structure dynamics and interplay : by single-particle electron microscopy
- Removal of Divalent Cations Induces Structural Transitions in Red Clover Necrotic Mosaic Virus, Revealing a Potential Mechanism for RNA Release
- Ab initio resolution measurement for single particle structures.
- Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex.
- Structural basis for actin assembly, activation of ATP hydrolysis, and delayed phosphate release.
- Reconstructing virus structures from nanometer to near-atomic resolutions with cryo-electron microscopy and tomography
- CryoEM and Molecular Dynamics of the Circadian KaiB–KaiC Complex Indicates KaiB Monomers Interact with KaiC and Block ATP Binding Clefts
- Determination of protein structure at 8.5Å resolution using cryo-electron tomography and sub-tomogram averaging.
- Structure of the T4 baseplate and its function in triggering sheath contraction
- Structural basis of small-molecule inhibition of human multidrug transporter ABCG2
- Structural insight into the assembly of the type II secretion system pilotin–secretin complex from enterotoxigenic Escherichia coli
- Structure of the human voltage-gated sodium channel Nav1.4 in complex with β1
- The complete structure of the human TFIIH core complex
- Structure of the activated human minor spliceosome
- Visualizing molecular machines in action: Single-particle analysis with structural variability.
- Cryoelectron microscopy of icosahedral virus particles.
- Three-Dimensional Asymmetric Reconstruction of Tailed Bacteriophage
- Blind deconvolution in scientific imaging & computational photography
- High-resolution views of TRPV1 and their implications for the TRP channel superfamily
- Single particle analysis at high resolution.
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