Extended polypeptide linkers establish the spatial architecture of a pyruvate dehydrogenase multienzyme complex.
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Summary
Using a combination of biochemical, biophysical, and cryo-electron microscopic techniques, it is shown that the gap between the E2 core and the shell of peripheral enzymes is maintained by the flexible but extended conformation adopted by 60 linker polypeptides that radiate outwards from the inner E2core, irrespective of the E1 or E3 occupancy.
- Type
- article
- Published
- 2008-01-01
- Cited by
- 25
- References
- 61
- Access
- Open access
- OpenAlex
- https://openalex.org/W18184587
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:34379840
Keywords
China, Sustenance, Foodways, Socioeconomic status, Geography
References
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- Cryoelectron microscopy of mammalian pyruvate dehydrogenase complex.
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- Ximdisp--A visualization tool to aid structure determination from electron microscope images.
Cited by
- The 2-oxoacid dehydrogenase multienzyme complex from thermophilic Archaea
- Snapshots of Catalysis in the E1 Subunit of the Pyruvate Dehydrogenase Multienzyme Complex
- Inter-chain Acetyl Transfer in the E2 Component of Bacterial Pyruvate Dehydrogenase Suggests a Model with Different Roles for Each Chain in a Trimer of the Homooligomeric Component
- Theoretical study of the catalytic mechanism of E1 subunit of pyruvate dehydrogenase multienzyme complex from Bacillus stearothermophilus.
- Protein-Protein Interactions in Assembly of Lipoic Acid on the 2-Oxoacid Dehydrogenases of Aerobic Metabolism*
- Nuclear Magnetic Resonance Approaches in the Study of 2-Oxo Acid Dehydrogenase Multienzyme Complexes—A Literature Review
- Order within disorder: Aggrecan chondroitin sulphate-attachment region provides new structural insights into protein sequences classified as disordered
- Localization of Inhibitory Antibodies to the Biotin Domain of Human Pyruvate Carboxylase
- Fab-PEG-Fab as a potential antibody mimetic.
- Salt-Induced Changes in the Subunit Structure of the Bacillus stearothermophilus Lipoate Acetyltransferase
- The E2 Domain of OdhA of Corynebacterium glutamicum Has Succinyltransferase Activity Dependent on Lipoyl Residues of the Acetyltransferase AceF
- Cotranslational assembly imposes evolutionary constraints on homomeric proteins
- Recent progress in multienzymes co-immobilization and multienzyme system applications
- Cotranslational protein assembly imposes evolutionary constraints on homomeric proteins
- The N-terminal 1-55 residues domain of pyruvate dehydrogenase from Escherichia coli assembles as a dimer in solution.
- The mannose phosphotransferase system (Man-PTS) - Mannose transporter and receptor for bacteriocins and bacteriophages.
- Unstructured regions of large enzymatic complexes control the availability of metabolites with signaling functions
- Integrative structure of a 10-megadalton eukaryotic pyruvate dehydrogenase complex from native cell extracts.
- MRPS36 provides a missing link in the eukaryotic 2-oxoglutarate dehydrogenase complex for recruitment of E3 to the E2 core
- MRPS36 provides a structural link in the eukaryotic 2-oxoglutarate dehydrogenase complex
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