Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.
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Summary
A dominant behavioral marker, rol‐6(su‐1006), and an efficient microinjection procedure which facilitate the recovery of Caenorhabditis elegans transformants are described and it is shown that low copy number extrachromosomal transformation can be achieved by adjusting the relative concentration of DNA molecules in the injection mixture.
- Type
- article
- Published
- 1991-12-01
- Cited by
- 3,226
- References
- 8
- Access
- Open access
- OpenAlex
- https://openalex.org/W2122163057
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:13869326
Keywords
Biology
References
Cited by
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- Control of cell fate in C. elegans by a GLP-1 peptide consisting primarily of ankyrin repeats
- Lineage-specific regulators couple cell lineage asymmetry to the transcription of the Caenorhabditis elegans POU gene unc-86 during neurogenesis.
- The SL1 trans-spliced leader RNA performs an essential embryonic function in Caenorhabditis elegans that can also be supplied by SL2 RNA.
- Structure and Expression of the Caenorhabditis elegans Protein Kinase C2 Gene
- Genetic control of programmed cell death and aging in the nematode Caenorhabditis elegans.
- Sperm-mediated gene transfer in the silkworm Bombyx mori.
- Differential Expression of Individual Suppressor tRNATrp Gene Family Members In Vitro and In Vivo in the Nematode Caenorhabditis elegans
- The VAB-1 Eph receptor tyrosine kinase functions in neural and epithelial morphogenesis in C. elegans.
- clr-1 encodes a receptor tyrosine phosphatase that negatively regulates an FGF receptor signaling pathway in Caenorhabditis elegans.
- Pioneer axon guidance by UNC-129, a C. elegans TGF-beta.
- Potential Roles of Peroxidases in Caenorhabditis Elegans Innate Immunity
- The LIM homeobox gene ceh-14 confers thermosensory function to the AFD neurons in Caenorhabditis elegans.
- Transgenic organisms in evolutionary ecology.
- Regulation of presynaptic terminal organization by C. elegans RPM-1, a putative guanine nucleotide exchanger with a RING-H2 finger domain.
- Cathepsin L Is Essential for Embryogenesis and Development ofCaenorhabditis elegans *
- Transgene expression in Strongyloides stercoralis following gonadal microinjection of DNA constructs.
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