Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.

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Summary

A dominant behavioral marker, rol‐6(su‐1006), and an efficient microinjection procedure which facilitate the recovery of Caenorhabditis elegans transformants are described and it is shown that low copy number extrachromosomal transformation can be achieved by adjusting the relative concentration of DNA molecules in the injection mixture.

Type
article
Published
1991-12-01
Cited by
3,226
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Open access

Keywords

Biology

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