Quantitative determination of serum triglycerides by the use of enzymes.
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Summary
A novel method for determining serum triglycerides, in which an enzymatic hydrolysis replaces the more commonly used saponification procedure, which is simple, rapid, and requires only 50 µl or less of sample.
- Type
- article
- Published
- 1973-05-01
- Cited by
- 3,517
- References
- 21
- Access
- Open access
- OpenAlex
- https://openalex.org/W2118313918
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:16497028
Keywords
Hydrolysis, Chromatography, Chemistry, Saponification, Glycerol
References
- The specificity of pancreatic lipase for the primary hydroxyl groups of glycerides.
- STUDIES ON LIPASE:IV. PURIFICATION AND PROPERTIES OF A LIPASE SECRETED BY RHIZOPUS DELEMAR
- The digestion in vitro of triglycerides by pancreatic lipase.
- Hydrolysis of primary and secondary esters of glycerol by pancreatic juice.
- Clinical Chemistry: Principles and Technics
- Determination of glycerides in blood serum.
- An improved method for determination of serum and plasma triglycerides.
- Application of thin-layer chromatography to the quantitation of plasma neutral lipids and free fatty acids.
- Simplified manual micromethod for determination of serum triglycerides.
- A simple method for the isolation and purification of total lipides from animal tissues.
- Complete separation of lipid classes on a single thin-layer plate.
- The Enzymes
- Triglycerides (“Neutral Fat”) in Serum*
Cited by
- Lipid and apolipoprotein levels during therapy with pinacidil combined with hydrochlorothiazide
- Familial high‐density‐lipoprotein deficiency causing corneal opacities (fish eye disease) in a family of Dutch descent
- The effects of low-dose Bay-m-1099 (Miglitol) on serum lipids and liver enzyme activity of obese and obese-diabetic corpulent rats.
- Fatty acid kinetics in man during chronic and acute illness.
- Normal serum response to oral beta-carotene in humans.
- Compared effect of n-3 and n-6 dietary fatty acids on rat intestinal acyl-CoA:cholesterol acyltransferase activity.
- Follow-up of subjects who developed chloracne following TCDD exposure at Seveso.
- Effect of mevinolin on cholesterol metabolism in obese and lean Zucker rats.
- In vitro metabolism of apolipoprotein E.
- Relative value of plasma copper, zinc, lipids and lipoproteins as markers for coronary artery disease.
- Effect of sulfonylurea therapy on plasma lipids and high-density lipoprotein composition in non-insulin-dependent diabetes mellitus.
- The anti-platelet effect of niceritrol in patients with arteriosclerosis and the relationship of the lipid-lowering effect to the anti-platelet effect.
- Lipid analysis in non-fasting diabetics.
- Relation between estrogen-induced hyperlipemia and food intake and body weight in rats.
- A weight reduction intervention that optimizes use of practitioner's time, lowers glucose level, and raises HDL cholesterol level in older adults.
- Apolipoproteins and ischaemic heart disease: implications for screening.
- Efficacy of Psyllium in Reducing Serum Cholesterol Levels in Hypercholesterolemic Patients on High- or Low-Fat Diets
- Lack of beta 3-adrenergic effect on lipolysis in human subcutaneous adipose tissue.
- Influence of particle size and sources of non-starch polysaccharides on postprandial glycaemia, insulinaemia and triacylglycerolaemia in pigs and starch digestion in vitro
- Independent and Interactive Association of Blood Antioxidants and Oxidative Damage in Elderly People
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