Microcolony Cultivation on a Soil Substrate Membrane System Selects for Previously Uncultured Soil Bacteria
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Summary
A novel microcultivation method for soil bacteria that mimics natural conditions and successful growth of soil bacteria as microcolonies confirmed that the missing culturable majority may have a growth strategy that is not observed when traditional cultivation indicators are used.
- Type
- article
- Published
- 2005-12-01
- Cited by
- 236
- References
- 35
- Access
- Open access
- OpenAlex
- https://openalex.org/W2115831350
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:6585745
Keywords
Bacteria, Soil bacteria, Biology, Biofilm, Soil microbiology
References
- The 'K' selected oligophilic bacteria: a key to uncultured diversity?
- Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity
- Nucleic acid techniques in bacterial systematics
- Microbial diversity in soil: the need for a combined approach using molecular and cultivation techniques.
- 16S/23S rRNA sequencing
- Viability of Indigenous Soil Bacteria Assayed by Respiratory Activity and Growth
- Modern Soil Microbiology
- Massilia timonae gen. nov., sp. nov., Isolated from Blood of an Immunocompromised Patient with Cerebellar Lesions
- Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology
- Cultivation of globally distributed soil bacteria from phylogenetic lineages previously only detected in cultivation-independent surveys.
- Cultivating the uncultured
- Diverse, yet-to-be-cultured members of the Rubrobacter subdivision of the Actinobacteria are widespread in Australian arid soils.
- Techniques with low hardware complexity of the driver electronics
- Rapid purification of DNA from soil for molecular biodiversity analysis
- Bacterial Identification for Publication: When Is Enough Enough?
- Use of Stable-Isotope Probing, Full-Cycle rRNA Analysis, and Fluorescence In Situ Hybridization-Microautoradiography To Study a Methanol-Fed Denitrifying Microbial Community
- Ribosomal RNA content in microcolony forming soil bacteria measured by quantitative 16S rRNA hybridization and image analysis
- A molecular approach to search for diversity among bacteria in the environment
- Cultivation of the ubiquitous SAR11 marine bacterioplankton clade
- Isolating "Uncultivable" Microorganisms in Pure Culture in a Simulated Natural Environment
Cited by
- Culturing marine bacteria – an essential prerequisite for biodiscovery
- Flow cytometry in environmental microbiology: a rapid approach for the isolation of single cells for advanced molecular biology analysis.
- Are Uncultivated Bacteria Really Uncultivable?
- Present and Future of Culturing Bacteria.
- Smartphone-based rapid quantification of viable bacteria by single-cell microdroplet turbidity imaging.
- Etude de bioremédiation de sédiments contaminés par des composés organiques nitrés persistants
- Caractérisation des communautés procaryotiques impliquées dans la bioremédiation d'un sol pollué par des hydrocarbures et développement d'outils d'analyse à haut débit : les biopuces ADN
- Microdroplet-enabled co-cultivation and characterization of microbial communities
- Culture-Independent and Culture-Dependent Approaches on Microbial Community Analysis at Gedongsongo (GS-2) Hot Spring
- Biodiversity, chemical diversity and drug discovery.
- Prokaryotic diversity of Boiling Springs Lake, Lassen Volcanic National Park
- Composition et fonctionnement d'une communauté microbienne au sein d'un drainage minier acide : approches culturales et fonctionnelles
- Isolation of previously uncultivable bacteria from a nickel contaminated soil using a diffusion-chamber-based approach
- Review and Future Development of New Culture Methods for Unculturable Soil Bacteria
- Towards the biogeography of British soil microorganisms
- Identification of Environmental Alphaproteobacteria with Conserved Signature Proteins in Metagenomic Datasets
- Better together: engineering and application of microbial symbioses.
- Biodégradation des herbicides en sols tempérés - Contrôle des communautés bactériennes dégradantes par la bioturbation du sol
- Application of a Bayesian nonparametric model to derive toxicity estimates based on the response of Antarctic microbial communities to fuel-contaminated soil
- Strategies for culture of 'unculturable' bacteria.
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