Active site mapping, biochemical properties and subcellular localization of rhodesain, the major cysteine protease of Trypanosoma brucei rhodesiense.
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Summary
This work expressed and purified rhodesain, the target protease of Trypanosoma brucei rhodesiense (MVAT4 strain), in reagent quantities from Pichia pastoris, and identified an invariable ER(A/V)FNAA motif in the pro-peptide sequence of r Rhodesain that was identified as being unique to the genus Trypano-Brucei.
- Type
- article
- Published
- 2001-11-01
- Cited by
- 160
- References
- 62
- OpenAlex
- https://openalex.org/W2106220465
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10206537
Keywords
Biology, Trypanosoma brucei, Protease, Biochemistry, Cysteine protease
References
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Cited by
- A Parasite Cysteine Protease Is Key to Host Protein Degradation and Iron Acquisition
- Michael acceptor based antiplasmodial and antitrypanosomal cysteine protease inhibitors with unusual amino acids.
- Cloning of a cDNA encoding Schistosoma mansoni cathepsin C and expression in the yeast Pichia pastoris
- RECOMBINANT EXPRESSION OF, AND CHARACTERISATION OF ANTIBODIES AGAINST VARIABLE SURFACE GLYCOPROTEINS: LITAT 1.3 AND LITAT 1.5 OF TRYPANOSOMA BRUCEI GAMBIENSE
- Recombinant expression, purification and characterisation of TviCATL for antibody production and diagnosis of African animal Trypanosomiasis.
- Biological Roles of Peptidases in Trypanosomatids
- Structural studies aimed at improving the antigenicity of congopain.
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- The propeptide of cruzipain − a potent selective inhibitor of the trypanosomal enzymes cruzipain and brucipain, and of the human enzyme cathepsin F
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- The cysteine proteinase inhibitor Z-Phe-Ala-CHN2 alters cell morphology and cell division activity of Trypanosoma brucei bloodstream forms in vivo
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- Screening and Identification of Inhibitors of Trypanosoma brucei Cathepsin L with Antitrypanosomal Activity
- Stage-specific translational efficiency and protein stability regulate the developmental expression of p37, an RNA binding protein from Trypanosoma brucei.
- RNA Interference of Trypanosoma brucei Cathepsin B and L Affects Disease Progression in a Mouse Model
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