Towards unbiased 3D reconstruction : in single-particle cryo-electron microscopy
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Summary
Cryo-electron microscopy of freestanding molecules (single-particles) plays a pivotal role in the difficult and pressing challenge of determining the structures of large macromolecular complexes.
- Type
- article
- Published
- 2010-01-01
- Cited by
- 1
- References
- 95
- Access
- Open access
- OpenAlex
- https://openalex.org/W611690605
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:82624455
Keywords
Cryo-electron microscopy, Electron microscope, Microscopy, Particle (ecology), Single particle analysis
References
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- On the correction of the contrast transfer function in biological electron microscopy
- Exact filters for general geometry three dimensional reconstruction
- Three-Dimensional Electron Microscopy of Macromolecular Assemblies
- Convergence of a Simulated Annealing Algorithm for Continuous Global Optimization
- Hierarchical wavelets projection matching for orientation determination of low contrast electron cryomicroscopic images of icosahedral virus particles
- Conformational changes studied by cryo-electron microscopy
- Differential Evolution – A Simple and Efficient Heuristic for global Optimization over Continuous Spaces
- Visualization of macromolecular structures
- α-Helical nascent polypeptide chains visualized within distinct regions of the ribosomal exit tunnel
- Structure of a chaperonin complex with a newly folded protein encapsulated in the folding chamber
- Dynamics of EF-G interaction with the ribosome explored by classification of a heterogeneous cryo-EM dataset.
- Classification of heterogeneous electron microscopic projections into homogeneous subsets.
- Noise bias in the refinement of structures derived from single particles.
- Structural Insight Into Nascent Polypeptide Chain-Mediated Translational Stalling
- Simulated tempering: a new Monte Carlo scheme
- Three‐dimensional reconstruction from a single‐exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli
- A common-lines based method for determining orientations for N > 3 particle projections simultaneously.
- Cross-correlation of common lines: a novel approach for single-particle reconstruction of a structure containing a flexible domain.
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- Future prospects
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