A simple technique for quantitation of low levels of DNA damage in individual cells.
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Summary
Human lymphocytes were exposed to X-irradiation or treated with H2O2 and the extent of DNA migration was measured using a single-cell microgel electrophoresis technique under alkaline conditions and this technique appears to be sensitive and useful for detecting damage and repair in single cells.
- Type
- article
- Published
- 1988-03-01
- Cited by
- 11,137
- References
- 10
- Access
- Open access
- OpenAlex
- https://openalex.org/W2092629964
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:520509
Keywords
Biology, DNA, DNA damage, DNA repair, Molecular biology
References
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- Mission to planet Earth.
- Differentiation of apurinic/apyrimidinic sites and single-strand breaks in DNA by formamide- and alkaline-sucrose gradient sedimentation.
- Comparative studies of DNA size in various tissues of mice during the aging process.
- Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells.
- X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution.
- Studies on the stability of trialkyl phosphates and di-(2'deoxythymidine) phosphotriesters in alkaline and neutral solution. A model study for hydrolysis of phosphotriesters in DNA and on the influence of a beta hydroxyethyl ester group.
- DNA repair synthesis in subpopulations of human lymphocytes.
- Dietary carcinogens and anticarcinogens. Oxygen radicals and degenerative diseases.
Cited by
- Systemic genotoxic effects of tobacco-related nitrosamines following oral and inhalational administration to Sprague-Dawley rats
- Single-cell gel electrophoresis (Comet) assay in nano-genotoxicology.
- Comparison of effects of iron and calcium chelators on the response of L5178Y sublines to X-rays and H2O2.
- Heterocyclic Aromatic Amines in Muscle Foods Relevance, Occurence and Mitigation
- The comet moment as a measure of DNA damage in the comet assay.
- Alkaline single‐cell gel (comet) assay and genotoxicity monitoring using two species of tadpoles
- Cytogenetic effects of butadiene metabolites in rat and mouse splenocytes following in vitro exposures.
- Induction of DNA damage by risk factors of colon cancer in human colon cells derived from biopsies.
- Evaluation of Storage Conditions for Assessing DNA Damage Using the Comet Assay
- Effects of smoking and aging on oxidative DNA damage of human lymphocytes.
- Comet assay detects cold repair of UV-A damages in a human B-lymphoblast cell line.
- Biomarker assessment of the effects of coal strip-mine contamination on channel catfish.
- Assessment of DNA damage in nurses handling antineoplastic drugs by the alkaline COMET assay.
- Evaluation of genotoxic and cytotoxic properties of pesticides employed in Italian agricultural practices.
- Protective role of probiotics and prebiotics in colon cancer.
- Role of reactive oxygen species in cupric 8-quinolinoxide-induced genotoxic effect.
- Evaluation of the cytotoxicity, mutagenicity and antimutagenicity of emerging edible plants.
- In vitro genotoxicity of terbutryn evaluated by the alkaline single-cell microgel-electrophoresis "comet" assay
- Lead induced DNA strand breaks in lymphocytes of exposed workers: role of reactive oxygen species and protein kinase C.
- Fusion Tyrosine Kinases Induce Drug Resistance by Stimulation of Homology-Dependent Recombination Repair, Prolongation of G2/M Phase, and Protection from Apoptosis
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