On the influence of vector design on antibody phage display.
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Summary
This study demonstrates simultaneously optimization of multiple and independent features of importance for phage selection, and concludes that removal of a 56 base pair fragment from the promoter region resulted in increased display level and increased virion production.
- Type
- article
- Published
- 2007-01-20
- Cited by
- 90
- References
- 43
- Access
- Open access
- OpenAlex
- https://openalex.org/W2084771912
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:25854403
Keywords
Phagemid, Phage display, Monoclonal antibody, Biology, Peptide library
References
- Aspects of the growth and regulation of the filamentous phages.
- Selection of human antibodies from phage display libraries.
- Screening of phage antibody libraries.
- Phage surface expression for analysis of recognition sites of human autoantibodies: Comparison of single chain Fv and Fab
- Phage Display: A Laboratory Manual
- Production of single-stranded plasmid DNA.
- A helper phage to improve single-chain antibody presentation in phage display
- Isolation of high affinity human antibodies directly from large synthetic repertoires.
- Modulation of antibody display on M13 filamentous phage.
- Antibodies from phage antibody libraries.
- Mating antibody phage display with proteomics.
- Parameters affecting the display of antibodies on phage.
- Human Antibodies with Sub-nanomolar Affinities Isolated from a Large Non-immunized Phage Display Library
- A phagemid vector using the E. coli phage shock promoter facilitates phage display of toxic proteins.
- A new helper phage and phagemid vector system improves viral display of antibody Fab fragments and avoids propagation of insert-less virions.
- Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.
- Continuous cultures of fused cells secreting antibody of predefined specificity
- Antibody-selectable filamentous fd phage vectors: affinity purification of target genes.
- Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p.
- The remarkable flexibility of the human antibody repertoire; isolation of over one thousand different antibodies to a single protein, BLyS.
Cited by
- Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV)
- Selection of recombinant antibodies from antibody gene libraries.
- Development of Escherichia coli cell surface display for selection of single domain antibodies from immune libraries
- Construction of human antibody gene libraries and selection of antibodies by phage display.
- Identification of a new epitope for HIV‐neutralizing antibodies in the gp41 membrane proximal external region by an Env‐tailored phage display library
- A new helper phage for improved monovalent display of Fab molecules.
- Progress in phage display: evolution of the technique and its applications
- In vivo Elimination of Parental Clones in General and Site-directed Mutagenesis
- Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display
- Single chain Fab (scFab) fragment
- Bacteriophage Vehicles for Phage Display: Biology, Mechanism, and Application
- The selection performance of an antibody library displayed on filamentous phage coat proteins p9, p3 and truncated p3
- Establishment of a reliable dual-vector system for the phage display of antibody fragments.
- Towards proteome scale antibody selections using phage display.
- SRP and Sec pathway leader peptides for antibody phage display and antibody fragment production in E. coli.
- Development of neutralizing scFv-Fc against botulinum neurotoxin A light chain from a macaque immune library
- A human scFv antibody generation pipeline for proteome research.
- A strategy for high-level expression of a single-chain variable fragment against TNFα by subcloning antibody variable regions from the phage display vector pCANTAB 5E into pBV220.
- Phage display-based identification and potential diagnostic application of novel antigens from Mycoplasma mycoides subsp. mycoides small colony type.
- Improved Fab presentation on phage surface with the use of molecular chaperone coplasmid system.
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