High hydrostatic pressure approach proves RNA catalytic activity without magnesium.
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Summary
It is shown that hammerhead ribozyme specifically cleaves RNA substrate at HHP in the absence of Mg(2+), which points out that the hydrolytic activity of nucleic acid depends on proper tertiary structure of a complex with a substrate.
- Type
- article
- Published
- 2007-06-01
- Cited by
- 18
- References
- 52
- OpenAlex
- https://openalex.org/W2058706574
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:39873858
Keywords
Deoxyribozyme, Nucleic acid, Ribozyme, RNA, Chemistry
References
- High pressure effects on biological macromolecules: from structural changes to alteration of cellular processes.
- Artificial tertiary motifs stabilize trans-cleaving hammerhead ribozymes under conditions of submillimolar divalent ions and high temperatures.
- Red blood cell magnesium concentrations: analytical problems and significance.
- The role of water structure in conformational changes of nucleic acids in ambient and high-pressure conditions.
- Hydrostatic and osmotic pressure study of the hairpin ribozyme.
- A simple two-structure model for liquid water
- Kinetics and thermodynamics of intermolecular catalysis by hairpin ribozymes.
- SURVEY AND SUMMARY Recent advances in the elucidation of the mechanisms of action of ribozymes
- Hydrolysis of oligoribonucleotides: influence of sequence and length.
- Capture and visualization of a catalytic RNA enzyme-product complex using crystal lattice trapping and X-ray holographic reconstruction.
- Evidence for the role of solvated metal hydroxide in the hammerhead cleavage mechanism.
- The decisive role of the water structure in changes of conformation of nucleic acids.
- High salt solution structure of a left-handed RNA double helix.
- Selection of Hammerhead Ribozyme Variants with Low Mg2+ Requirement: Importance of Stem‐Loop II
- Ca2+-dependent Modulation of Intracellular Mg2+ Concentration with Amiloride and KB-R7943 in Pig Carotid Artery*
- Hairpin Ribozyme-Catalyzed Ligation in Water-Alcohol Solutions
- Nonenzymatic hydrolysis of oligoribonucleotides.
- A-Z-RNA conformational changes effected by high pressure.
- Fast cleavage kinetics of a natural hammerhead ribozyme.
- A nature of conformational changes of yeast tRNA(Phe). High hydrostatic pressure effects.
Cited by
- Catalytic DNAzymes: derivations and functions
- Adenine, a hairpin ribozyme cofactor – high‐pressure and competition studies
- Facilitation of RNA enzyme activity in the molecular crowding media of cosolutes.
- Effect of high hydrostatic pressure on hydration and activity of ribozymes
- The hydrogen bond under pressure
- Highly active low magnesium hammerhead ribozyme.
- The mechanism of acidic hydrolysis of esters explains the HDV ribozyme activity
- Probing fast ribozyme reactions under biological conditions with rapid quench-flow kinetics
- Elucidation of pressure-induced lid movement and catalysis behavior of Rhizopus chinensis lipase.
- Nucleic Acid Catalysis under Potential Prebiotic Conditions
- Ribozyme Chemistry: To Be or Not To Be under High Pressure.
- Organic acids under pressure: elastic properties, negative mechanical phenomena and pressure induced phase transitions in the lactic, maleic, succinic and citric acids
- Ultrafast dynamics under high-pressure
- Pressure pushes tRNALys3 into excited conformational states
- Modularity, ecology, and theoretical evolution of the ribozyme body plan
- NUCLEIC ACID CATALYSTS: INVESTIGATING THE EFFECT OF MONOVALENT IONS ON THE ACTIVITY OF DNA ENZYMES
- ACID CATALYSTS : COMPARING THE MECHANISMS OF DNA AND RNA ENZYMES
- RNA Technologies for Mitochondrial Genetics
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