Class-IIS restriction enzymes--a review.
Explore this paper's citation graph
Summary
Many unique applications of this class of enzymes are described, including: precise trimming of DNA; retrieval of cloned fragments; gene assembly; use as a universal restriction enzyme; cleavage of single-stranded DNA; detection of point mutations; tandem amplification; printing-amplification reaction; and localization of methylated bases.
- Type
- review
- Published
- 1991-04-01
- Cited by
- 246
- References
- 95
- OpenAlex
- https://openalex.org/W2044069937
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:43415464
Keywords
Biology, Restriction enzyme, Isoschizomer, Recognition sequence, DNA
References
- Cleavage specificity of the restriction endonuclease isolated from Haemophilus gallinarum (Hga I).
- Boundaries of the nutL antiterminator of coliphage lambda and effects of mutations in the spacer region between boxA and boxB.
- Synthesis of the human insulin gene. Part IV. New synthetic deoxyribooligonucleotide adaptors and primer for DNA cloning and sequence analysis.
- RleAI: a novel class-IIS restriction endonuclease from Rhizobium leguminosarum recognizing 5'-CCCACA(N)12-3' 3'-GGGTGT(N)9-5'.
- Ribonuclease P: an enzyme with a catalytic RNA subunit.
- The FokI restriction-modification system. II. Presence of two domains in FokI methylase responsible for modification of different DNA strands.
- The fokI restriction-modification system. I. Organization and nucleotide sequences of the restriction and modification genes.
- Type III 5-methylcytosine modification of DNA in Neisseria gonorrhoeae
- Taxonomic specificity of restriction-modification enzymes.
- Nomenclature for bacterial genes coding for class-II restriction endonucleases and modification methyltransferases.
- Insertional mutagenesis using a synthetic lac operator.
- Conferring operator specificity on restriction endonucleases.
- Amplification of cloned DNA as tandem multimers using BspMI-generated asymmetric cohesive ends.
- Sequence specificity of DNA methylases from Bacillus amyloliquefaciens and Bacillus brevis.
- Molecular structure of a left-handed double helical DNA fragment at atomic resolution
- Two sequence-specific endonucleases from Moraxella bovis.
- A cloning vector allowing excision of inserts with original termini irrespective of their sequence.
- A new type II restriction endonuclease, BsmA I, from Bacillus stearothermophilus.
- A second site specific endonuclease from Thermus thermophilus 111, Tth111II.
- Ksp632I, a novel class-IIS restriction endonuclease from Kluyvera sp. strain 632 with the asymmetric hexanucleotide recognition sequence: 5'-CTCTTC(N)1-3' 3'-GAGAAG(N)4-5'.
Cited by
- Isolation and characterization of new restriction endonucleases from Vibrio parahaemolyticus: VpaK32I enzyme with the class-IIS heptanucleotide specificity, GCTCTTCN1/N4.
- Generation of families of construct variants using golden gate shuffling.
- High efficiency DNA mutagenesis mediated by using in vitro transcription, DNase I digestion, and RT-PCR.
- A simple method for sequencing small DNAs by introducing precise overlapping ends into restriction digestion fragments.
- DNA hydrolysis and genome editing applications of GIY-YIG family homing endonucleases
- Novel Subtype of Type IIs Restriction Enzymes
- Mutational Analysis of Two Putative Catalytic Motifs of the Type IV Restriction Endonuclease Eco57I*
- Designing zinc finger nucleases that specifically cleave Hepatitis B viral DNA
- REPSA: combinatorial approach for identifying preferred drug-DNA binding sequences.
- Assembly of Highly Asymmetric Genetically-Encoded Amphiphiles for Thermally Targeted Delivery of Therapeutics
- DNA splicing by directed ligation (SDL).
- Methylation of DNA in prokaryotes.
- Molecular cloning using the interrupted recognition sequence of BplI to select for recombinant molecules.
- Function and application of TAL effectors
- The Unique FauI Restriction–Modification System: Cloning and Protein Sequence Comparisons
- Combinatorial DNA assembly using Golden Gate cloning.
- MboII endonuclease heat inactivation before agarose gel electrophoresis to prevent artifactual bands in restriction patterns.
- Restriction Enzymes in Microbiology, Biotechnology and Biochemistry
- FX cloning: a versatile high-throughput cloning system for characterization of enzyme variants.
- M.BstF5I-4, the Forth DNA-Methyltransferase of the BstF5I Restriction–Modification System from Bacillus stearothermophilus F5
Related papers
- [BsiI--a new unusual restriction endonuclease].
- [Substrate specificity of restriction endonuclease Eco781].
- Type II restriction endonucleases from Helicobacter pylori include an enzyme with a novel recognition sequence.
- Type II restriction endonucleases from Helicobacter pylori include an enzyme with a novel recognition sequence
- [Specificity of restriction endonuclease VneI].
- Inhibition of Restriction Enzyme's DNA Sequence Recognition by PUVA Treatment†,¶
- [Site-specific restriction endonuclease RME211 from Rhizobium meliloti].
- Bca77I: a new type-II restriction endonuclease from Bacillus caldolyticus cutting at the (A/T) increases CCGG(A/T) site.