Automated neurosphere sorting and plating by the COPAS large particle sorter is a suitable method for high-throughput 3D in vitro applications.
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Summary
The results of the comparison of NPC viability, proliferation, migration, differentiation and intracellular oxidative stress between manually and COPAS sorted and plated neurosphere of different species show that the automation by the COPAS instrument does not influence the basic performance of neurospheres.
- Type
- article
- Published
- 2012-09-01
- Cited by
- 23
- References
- 31
- OpenAlex
- https://openalex.org/W1970952227
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:33959363
Keywords
Neurosphere, Biology, Neural stem cell, Cell biology, In vitro
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Cited by
- Comparative human and rat neurospheres reveal species differences in chemical effects on neurodevelopmental key events
- ESNATS Conference — The use of Human Embryonic Stem Cells for Novel Toxicity Testing Approaches a
- Comparative Human and Rat “Neurosphere Assay” for Developmental Neurotoxicity Testing
- Challenges and opportunities toward enabling phenotypic screening of complex and 3D cell models.
- Cultured stem cells as tools for toxicological assays.
- Characterization of calcium responses and electrical activity in differentiating mouse neural progenitor cells in vitro.
- Biological and medical applications of a brain-on-a-chip
- Clusters of neural stem/progenitor cells cultured on a soft poly(vinyl alcohol) hydrogel crosslinked by gamma irradiation.
- In vitro acute and developmental neurotoxicity screening: an overview of cellular platforms and high-throughput technical possibilities
- Literature review on in vitro and alternative Developmental Neurotoxicity (DNT) testing methods
- Assessment of drug-loaded nanoparticles in a 3D in vitro brain tumour model
- Comparative performance analysis of human iPSC-derived and primary neural progenitor cells (NPC) grown as neurospheres in vitro.
- A transcriptome comparison of time‐matched developing human, mouse and rat neural progenitor cells reveals human uniqueness
- An industry perspective: A streamlined screening strategy using alternative models for chemical assessment of developmental neurotoxicity.
- Molekulare Untersuchungen zur Spezies-spezifischen Toxizität entwicklungsneurotoxischer Substanzen
- Establishment of an a priori protocol for the implementation and interpretation of an in‐vitro testing battery for the assessment of developmental neurotoxicity
- Human Induced Pluripotent Stem Cell‐Derived Neural Progenitor Cells Produce Distinct Neural 3D In Vitro Models Depending on Alginate/Gellan Gum/Laminin Hydrogel Blend Properties
- Position of the Kenzan Method in the Space-Time of Tissue Engineering
- Building Blocks for Bottom-Up Neural Tissue Engineering: Tools for In Vitro Assembly and Interrogation of Neural Circuits
- The Neurosphere Assay as an In Vitro Method for Developmental Neurotoxicity (DNT) Evaluation
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